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http://hdl.handle.net/1843/41917| Type: | Artigo de Periódico |
| Title: | Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes |
| Authors: | Michele Munk Pereira Ado Jorio de Vasconcelos Humberto de Mello Brandão Luiz Orlando Ladeira Bruno Campos de Carvalho Luiz Sérgio de Almeida Camargo Nádia Rezende Barbosa Raposo Raquel Varella Serapião Carolina Capobiango Romano Quintão Saulo Rodrigues Silva Jaqueline dos Santos Soares |
| Abstract: | The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos. |
| Subject: | Nanotubos DNA |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICX - DEPARTAMENTO DE FÍSICA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | 10.1038/srep33588 |
| URI: | http://hdl.handle.net/1843/41917 |
| Issue Date: | 19-Sep-2016 |
| metadata.dc.url.externa: | https://www.nature.com/articles/srep33588 |
| metadata.dc.relation.ispartof: | Scientific Reports |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Efficient delivery of DNA into.pdf | 2.03 MB | Adobe PDF | View/Open |
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