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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/42229
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dc.creatorJosiane dos Santospt_BR
dc.creatorAlessandra Rejane Ericsson de Oliveira Xavierpt_BR
dc.creatorMauro Aparecido de Sousa Xavierpt_BR
dc.creatorLéia Cardosopt_BR
dc.creatorSérgio Avelino Mota Nobrept_BR
dc.creatorRicardo Rodrigues Bacchipt_BR
dc.creatorCarlos Humbertp Chamone Cangussupt_BR
dc.creatorAnna Christina de Almeidapt_BR
dc.creatorLuciana Nobre Leitept_BR
dc.creatorNair Amélia Prates Barretopt_BR
dc.date.accessioned2022-06-03T15:57:27Z-
dc.date.available2022-06-03T15:57:27Z-
dc.date.issued2020-
dc.citation.volume19pt_BR
dc.citation.issue1pt_BR
dc.identifier.doihttps://doi.org/10.4238/gmr18521pt_BR
dc.identifier.issn1676-5680pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/42229-
dc.description.resumoPigeon droppings carry microorganisms such as Candida spp., which have high genetic variability and are the most common opportunistic pathogens in humans. There is a need to establish fast, reliable and simple laboratory methods to differentiate pathogenic from non-pathogenic yeast species in pigeon droppings, as they can be a source of contamination for humans. We examined yeasts isolated from domestic pigeon droppings through a proteomic approach using matrix-assisted laser desorption ionization (MALDI-TOF). We also examined genetic characteristics of the isolates by restriction fragment length polymorphism (RFLP) PCR and enterobacterial repetitive intergenic consensus sequences PCR methods. Five colonies isolated from domestic pigeon droppings collected at three different sampling locations on the roof of a Brazilian university building were identified as Candida krusei and Candida rugosa by MALDI-TOF. Phylogenetic studies using the two PCR-based molecular methods were able to differentiate the species C. krusei and C. rugosa into different groups. These genomic methods gave similar results and proved useful for genetic diversity analysis and interspecific differentiation of Candida isolates.pt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIASpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofGenetics and Molecular Researchpt_BR
dc.rightsAcesso Abertopt_BR
dc.subject.otherLevedospt_BR
dc.subject.otherFilogeniapt_BR
dc.titleIdentification and molecular analysis of yeast in droppings of domestic pigeons in Montes Claros, Brazil.pt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.geneticsmr.com/articles/identification-and-molecular-analysis-yeasts-found-domestic-pigeon-droppings-montes-clarospt_BR
dc.identifier.orcidhttps://orcid.org/ 0000-0001-9836-4117pt_BR
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