Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/42279
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dc.creatorGeziella Áurea Aparecida Damasceno Souzapt_BR
dc.creatorAnna Christina de Almeidapt_BR
dc.creatorMauro Aparecido de Sousa Xavierpt_BR
dc.creatorLívia Mara Vitorino da Silvapt_BR
dc.creatorCintya Neves Sousapt_BR
dc.creatorDemerson Arruda Sanglardpt_BR
dc.creatorAlessandra Rejane Ericsson de Oliveira Xavierpt_BR
dc.date.accessioned2022-06-06T15:48:42Z-
dc.date.available2022-06-06T15:48:42Z-
dc.date.issued2019-
dc.citation.volume12pt_BR
dc.citation.issue12pt_BR
dc.citation.spage1931pt_BR
dc.citation.epage1939pt_BR
dc.identifier.doiwww.doi.org/10.14202/vetworld.2019.1931-1939pt_BR
dc.identifier.issn2231-0916pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/42279-
dc.description.resumoBackground and aim: The term ESKAPE, recognized by the WHO, is an acronym, which refers to the pathogens Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp., which is extremely virulent and multidrug-resistant. Although the term is used to designate nosocomial pathogens, in a milking environment, strains of Methicillin-resistant S. aureus have been isolated from cattle diagnosed with clinical and subclinical mastitis. Resistant strains may be involved in the transfer of genes conferring resistance to beta-lactam antimicrobials among the species of microorganisms related to mastitis etiology. This study aimed to trace the phenotypic and genotypic profiles of susceptibility to beta-lactams in S. aureus isolated from milk of cattle diagnosed with subclinical mastitis obtained from different rural properties located in the North of Minas Gerais State, Brazil. Materials and methods: Sixteen microorganisms previously identified as S. aureus isolated from milk of cattle diagnosed with subclinical mastitis were submitted to matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF), mass spectrometry, and polymerase chain reaction (PCR) analysis for microbial species confirmation. The S. aureus beta-lactams antimicrobial phenotypic resistance profile was investigated by disk diffusion method. PCR methods were also performed to investigate the S. aureus genotypic beta-lactams resistance profile. For this purpose, bla Z, mec A, mec ALGA251, bla Oxa23, and bla KPC genes were screened among S. aureus isolates. The genetic diversity of S. aureus by fingerprint random amplified polymorphic DNA (RAPD)-PCR was also performed in this study. Results: All isolates showed phenotypic resistance to at least three beta-lactams, among which was meropenem. None of the isolates tested positive for the genes mec ALGA251, bla Oxa23, and bla KPC; however, the presence of the genes bla Z and mecA was detected among the isolates. The fingerprint analysis divided isolates into two distinct groups and 15 different subgroups. Despite the presence of clonality among the isolates, the PCR-RAPD analysis unveiled a heterogeneous profile with genetic diversity among the S. aureus isolates. Conclusion: In this study, we identified beta-lactams resistant S. aureus strains isolated from the milk of cows diagnosed with subclinical mastitis. The S. aureus beta-lactams resistance was investigated using a phenotypic and genotypic approach. We believe that molecular epidemiology, improved knowledge, and genetic basis of resistance to beta-lactams might assist in asserting guidelines for better management practices of dealing with subclinical mastitis and mapping of origin of resistant pathogens in the studied Brazilian area.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipOutra Agênciapt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIASpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofVeterinary Worldpt_BR
dc.rightsAcesso Abertopt_BR
dc.subject.otherStaphylococcus aureuspt_BR
dc.subject.otherAntibioticos beta-lactamicospt_BR
dc.subject.otherGenéticapt_BR
dc.subject.otherInfecçãopt_BR
dc.titleCharacterization and molecular epidemiology of staphylococcus aureus strains resistant to beta-lactams isolated from the milk of cows diagnosed with subclinical mastitispt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttp://www.veterinaryworld.org/Vol.12/December-2019/7.pdfpt_BR
dc.identifier.orcidhttps://orcid.org/ 0000-0001-9836-4117pt_BR
Appears in Collections:Artigo de Periódico



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