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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/42376
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dc.creatorEliane Macedosobrinho Santospt_BR
dc.creatorAnna Christina de Almeidapt_BR
dc.creatorHércules Otacílio Santospt_BR
dc.creatorAlexsander Rodrigues Cangussupt_BR
dc.creatorDeborah Aires Almeidapt_BR
dc.creatorKattyanne Souza Costapt_BR
dc.date.accessioned2022-06-09T11:06:03Z-
dc.date.available2022-06-09T11:06:03Z-
dc.date.issued2018-
dc.citation.volume80pt_BR
dc.citation.issue8pt_BR
dc.citation.spage1317pt_BR
dc.citation.epage1324pt_BR
dc.identifier.doihttp://doi.org/10.1292/jvms.16-0581pt_BR
dc.identifier.issn1347-7439pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/42376-
dc.description.resumoWe conducted an in silico analysis to search for important genes in the pathogenesis of Caseous Lymphadenitis (CL), with prospects for use in formulating effective vaccines against this disease. For this, we performed a survey of proteins expressed by Corynebacterium pseudotuberculosis, using protein sequences collected from the NCBI GenPept database and the keywords “caseous lymphadenitis” and “Corynebacterium pseudotuberculosis” and “goats”. A network was developed using the STRING 10 database, with a confidence score of 0.900. For every gene interaction identified, we summed the interaction score of each gene, generating a combined association score to obtain a single score named weighted number of links (WNL). Genes with the highest WNL were named “leader genes”. Ontological analysis was extracted from the STRING database through Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A search in the GenPept database revealed 2,124 proteins. By using and plotting with STRING 10, we then developed an in silico network model comprised of 1,243 genes/proteins interconnecting through 3,330 interactions. The highest WNL values were identified in the rplB gene, which was named the leader gene. Our ontological analysis shows that this protein acts effectively mainly on Metabolic pathways and Biosynthesis of secondary metabolites. In conclusion, the in silico analyses showed that rplB has good potential for vaccine development. However, functional assays are needed to make sure that this protein can potentially induce both humoral and cellular immune responses against C. pseudotuberculosis in goats.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorshipOutra Agênciapt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIASpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Veterinary Medical Sciencept_BR
dc.rightsAcesso Abertopt_BR
dc.subject.otherBacterias gram-positivaspt_BR
dc.subject.otherOntologias (Recuperação da informação)pt_BR
dc.subject.otherSimulação (Computadores)pt_BR
dc.subject.otherLinfadenite caseosa em caprinopt_BR
dc.titleLeader gene of corynebacterium pseudotuberculosis may be useful in vaccines against caseous lymphadenitis of goats: a bioinformatics approachpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://pubmed.ncbi.nlm.nih.gov/29937460/pt_BR
dc.identifier.orcidhttps://orcid.org/ 0000-0001-9836-4117pt_BR
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