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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/42419
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dc.creatorAlice Freitas Versianipt_BR
dc.creatorAdo Jorio de Vasconcelospt_BR
dc.creatorFlávio Guimarães da Fonsecapt_BR
dc.creatorRuiz Gerhardt Astigarragapt_BR
dc.creatorEliseu Soares de Oliveira Rochapt_BR
dc.creatorAna Paula Moreira Barbozapt_BR
dc.creatorErna Geessien Kroonpt_BR
dc.creatorMilene Alvarenga Rachidpt_BR
dc.creatorDaniele da Glória de Souzapt_BR
dc.creatorLuiz Orlando Ladeirapt_BR
dc.creatorEdel Figueiredo Barbosa Stanciolipt_BR
dc.date.accessioned2022-06-10T14:52:43Z-
dc.date.available2022-06-10T14:52:43Z-
dc.date.issued2017-04-04-
dc.citation.volume15pt_BR
dc.citation.spage1pt_BR
dc.citation.epage13pt_BR
dc.identifier.doihttps://doi.org/10.1186/s12951-017-0259-4pt_BR
dc.identifier.issn1477-3155pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/42419-
dc.description.resumoBackground: Dengue is the most prevalent arthropod‑borne viral disease in the world. In this article we present results on the development, characterization and immunogenic evaluation of an alternative vaccine candidate against Dengue. Methods: The MWNT‑DENV3E nanoconjugate was developed by covalent functionalization of carboxylated multi‑walled carbon nanotubes (MWNT) with recombinant dengue envelope (DENV3E) proteins. The recombinant antigens were bound to the MWNT using a diimide‑activated amidation process and the immunogen was characterized by TEM, AFM and Raman Spectroscopy. Furthermore, the immunogenicity of this vaccine candidate was evaluated in a murine model. Results: Immunization with MWNT‑DENV3E induced comparable IgG responses in relation to the immunization with non‑conjugated proteins; however, the inoculation of the nanoconjugate into mice generated higher titers of neutralizing antibodies. Cell‑mediated responses were also evaluated, and higher dengue‑specific splenocyte proliferation was observed in cell cultures derived from mice immunized with MWNT‑DENV3E when compared to animals immu‑ nized with the non‑conjugated DENV3E. Conclusions: Despite the recent licensure of the CYD‑TDV dengue vaccine in some countries, results from the vaccine’s phase III trial have cast doubts about its overall efficacy and global applicability. While questions about the effectiveness of the CYD‑TDV vaccine still lingers, it is wise to keep at hand an array of vaccine candidates, including alternative non‑classical approaches like the one presented here.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIApt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIApt_BR
dc.publisher.departmentICX - DEPARTAMENTO DE FÍSICApt_BR
dc.publisher.departmentVET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Nanobiotechnologypt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectDengue vaccinept_BR
dc.subjectCarbon nanotubespt_BR
dc.subjectSubunit vaccinept_BR
dc.subjectNanoconjugatept_BR
dc.subject.otherNanotubos de carbonopt_BR
dc.subject.otherEspectroscopia de Ramanpt_BR
dc.subject.otherDenguept_BR
dc.titleMulti-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in micept_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-017-0259-4pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-1378-5380pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-5978-2735pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-2854-0768pt_BR
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