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Type:	Artigo de Periódico
Title:	Anti-botulism single-shot vaccine using chitosan for protein encapsulation by simple coacervation
Authors:	Roger Shoji Sari Angelo Samir m. Migue Eliane Macedo Sobrinho Santos Anna Christina de Almeida Alex Sander Cangussu Edson Vander Jorge Otto Domenici Mozzer Hercules Otacilio Santos Wagner Quintilio Igor Viana Brandi Viviane Aguiar Andrade
Abstract:	The aim of the present study was to compare the potency and safety of vaccines against Clostridium botulinum (C. botulinum) type C and D formulated with chitosan as controlled release matrix and vaccines formulated in conventional manner using aluminum hydroxide. Parameters were established for the development of chitosan microspheres, using simple coacervation to standardize the use of this polymer in protein encapsulation for vaccine formulation. To formulate a single shot vaccine inactivated antigens of C. botulinum type C and D were used with original toxin titles equal to 5.2 and 6.2 log LD50/ml, respectively. For each antigen a chitosan based solution of 50 mL was prepared. Control vaccines were formulated by mixing toxoid type C and D with aluminum hydroxide [25% Al(OH)3, pH 6.3]. The toxoid sterility, innocuity and potency of vaccines were evaluated as stipulated by MAPA-BRASIL according to ministerial directive no. 23. Encapsulation efficiency of BSA in chitosan was 32.5e40.37%, while that the encapsulation efficiency to toxoid type C was 41,03% (1.94 mg/mL) and of the toxoid type D was 32.30% (1.82 mg/mL). The single shot vaccine formulated using chitosan for protein encapsulation through simple coacervation showed potency and safety similar to conventional vaccine currently used in Brazilian livestock (10 and 2 IU/mL against C. botulinum type C and D, respectively). The present work suggests that our single shot vaccine would be a good option as a cattle vaccine against these C. botulinum type C and D.
Subject:	Vacina veterinária Quitosana Antígenos Agentes nefrotóxicos
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
Rights:	Acesso Aberto
metadata.dc.identifier.doi:	https://doi.org/10.1016/j.anaerobe.2016.10.013
URI:	http://hdl.handle.net/1843/42502
Issue Date:	2016
metadata.dc.url.externa:	https://www.sciencedirect.com/science/article/pii/S1075996416301329
metadata.dc.relation.ispartof:	Anaerobe
Appears in Collections:	Artigo de Periódico

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