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http://hdl.handle.net/1843/43348| Type: | Artigo de Periódico |
| Title: | Differential cellular immune response of galleria mellonella to actinobacillus pleuropneumoniae |
| Other Titles: | Resposta imune celular diferencial de galleria mellonella a actinobacillus pleuropneumoniae |
| Authors: | Luis Andrésarteaga Blanco Josicelli Souza Crispim Kenner Morais Fernandes Leandro Licursi de Oliveira Monalessa Fábia Pereira Denise Mara Soares Bazzolli Gustavo Ferreira Martins |
| Abstract: | In the present work, we have investigate the cellular immune response of Galleria mellonella larvae against three strains of the gram-negative bacterium Actinobacillus pleuropneumoniae: low-virulence (780), high-virulence (1022) and the serotype 8 reference strain (R8). Prohemocytes, plasmatocytes, granulocytes, oenocytoids and spherulocytes were distinguished according to their size and morphology, their molecular markers and dye-staining properties and their role in the immune response. Total hemocyte count, differential hemocyte count, lysosome activity, autophagic response, cell viability and caspase-3 activation were determined in circulating hemocytes of naive and infected larvae. The presence of the autophagosome protein LC3 A/B within the circulating hemocytes of G. mellonella was dependent on and related to the infecting A. pleuropneumoniae strain and duration of infection. Hemocytes treated with the high-virulence strain expressed higher levels of LC3 A/B, whereas treatment with the low-virulence strain induced lower expression levels of this protein in the cells. Moreover, our results showed that apoptosis in circulating hemocytes of G. mellonella larvae after exposure to virulent bacterial strains occurred simultaneously with excessive cell death response induced by stress and subsequent caspase-3 activation. |
| Abstract: | No presente trabalho, investigamos a resposta imune celular de larvas de Galleria mellonella contra três cepas da bactéria gram-negativa Actinobacillus pleuropneumoniae: baixa virulência (780), alta virulência (1022) e a cepa de referência do sorotipo 8 (R8) . Prohemócitos, plasmatócitos, granulócitos, oenocitóides e esferulócitos foram distinguidos de acordo com seu tamanho e morfologia, seus marcadores moleculares e propriedades corantes e seu papel na resposta imune. Contagem total de hemócitos, contagem diferencial de hemócitos, atividade lisossômica, resposta autofágica, viabilidade celular e ativação de caspase-3 foram determinados em hemócitos circulantes de larvas virgens e infectadas. A presença da proteína autofagossômica LC3 A/B nos hemócitos circulantes de G. mellonella foi dependente e relacionada à cepa infectante de A. pleuropneumoniae e à duração da infecção. Hemócitos tratados com a cepa de alta virulência expressaram níveis mais elevados de LC3 A/B, enquanto o tratamento com a cepa de baixa virulência induziu níveis mais baixos de expressão dessa proteína nas células. Além disso, nossos resultados mostraram que a apoptose em hemócitos circulantes de larvas de G. mellonella após exposição a cepas bacterianas virulentas ocorreu simultaneamente com resposta excessiva de morte celular induzida por estresse e subsequente ativação da caspase-3. |
| Subject: | Larvas de Galleria mellonella Actinobacillus pleuropneumoniae Resposta Imune Celular |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | COLTEC - COLEGIO TECNICO |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | 10.1007/s00441-017-2653-5 |
| URI: | http://hdl.handle.net/1843/43348 |
| Issue Date: | 2017 |
| metadata.dc.url.externa: | https://link.springer.com/article/10.1007/s00441-017-2653-5 |
| metadata.dc.relation.ispartof: | Cell & tissue research |
| Appears in Collections: | Artigo de Periódico |
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