Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/44673
Type: Artigo de Periódico
Title: Evaluation of three different decontamination techniques on biofilm formation, and on physical and chemical properties of resin composites
Authors: Carolina Bosso André
Andressa dos Santos
Carmem Silvia Pfeifer
Marcelo Giannini
Emerson Marcelo Girotto
Jack Liborio Ferracane
Abstract: Objectives: This study evaluated three different sterilization/disinfection techniques for resin composites on bacterial growth and surface modification after decontamination. Methods: Two resin composites were sterilized/disinfected with three different techniques: UV light, 1% chloramine T, and 70% ethanol. Four different times were used for each technique to determine the shortest time that the solution or UV light was effective. The influence of sterilization/disinfection technique on bacterial growth was evaluated by analyzing the metabolic activity, using the AlamarBlue™ assay, bacterial viability, and SEM images from biofilms of Streptococcus mutans. The surface change, after the process, was analyzed with ATR/FTIR and SEM images. The solutions used for decontamination (1% chloramine-T and 70% ethanol) were analyzed with 1H-NMR to identify any resin compounds leached during the process. Results: One minute of decontamination was efficient for all three methods tested. Chloramine-T increased the surface porosity on resin composites, no changes were observed for UV light and 70% ethanol, however, 1H-NMR identified leached monomers only when 70% ethanol was used. No chemical change of the materials was found under ATR/FTIR analyses after the decontamination process. Chloramine-T, with no previous wash, increased the bacterial viability for both resin composites and increased the bacterial metabolism for the resin composite without fluoride. Conclusion: UV light had no interference on the resin composites properties tested using 1 min of exposure compared to the other decontamination methods
Subject: Composite resins
Sterilization
Biofilms
Disinfection
language: eng
metadata.dc.publisher.country: Brasil
Publisher: Universidade Federal de Minas Gerais
Publisher Initials: UFMG
metadata.dc.publisher.department: FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
Rights: Acesso Aberto
metadata.dc.identifier.doi: 10.1002/jbm.b.33907
URI: http://hdl.handle.net/1843/44673
Issue Date: Apr-2018
metadata.dc.url.externa: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381933/
metadata.dc.relation.ispartof: Journal of Biomedical materials research part b-applied biomaterials
Appears in Collections:Artigo de Periódico



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