1. Repositório Institucional da UFMG
  2. Publicações Científicas e Culturais
  3. Artigo de Periódico
Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/45492
Full metadata record
DC FieldValueLanguage
dc.creatorFrancine Benettipt_BR
dc.creatorAndré Luiz Fraga Brisopt_BR
dc.creatorAraújo Lopespt_BR
dc.creatorCarminattipt_BR
dc.creatorContipt_BR
dc.creatorMarjorie de Oliveira Gallinaript_BR
dc.creatorEdilson Ervolinopt_BR
dc.creatorLuciano Tavares Angelo Cintrapt_BR
dc.date.accessioned2022-09-24T20:53:45Z-
dc.date.available2022-09-24T20:53:45Z-
dc.date.issued2019-07-19-
dc.citation.volume52pt_BR
dc.citation.issue12pt_BR
dc.citation.spage1723pt_BR
dc.citation.epage1737pt_BR
dc.identifier.doihttps://doi.org/10.1111/iej.13190pt_BR
dc.identifier.issn01432885pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/45492-
dc.description.resumoAimTo investigate hydrogen peroxide (H2O2)-inducedresponsiveness in pulp cells using heme oxygenase-1(HO-1) immunolabelling, Jun-D immunolabelling tostudy the effects of H2O2on odontoblastic differentiationand CD90+/CD73+/CD105+/CD45 cell counting forin vivoidentification of mesenchymal stem cells in thepulp.MethodologyThe maxillary molars of 50 rats weretreated with a bleaching gel (35% H2O2,1930 min) orplacebo gel (control groups). At 2, 3, 7, 15 and 30 daysafter the treatment (n=10), inflammation in pulp tissuewas analysed by haematoxylin–eosin staining, HO-1-and Jun-D-immunolabelled cells were counted ineach third of the pulp chamber, and the number ofCD90+/CD73+/CD105+/CD45 cells was quantified byimmunofluorescence. The results were assessed using thePairedt-test or Wilcoxon signed-rank test (P<0.05).ResultsSignificant H2O2-induced inflammation wasnoted at 2 and 3 days (P<0.05), with tertiary den-tine formation occurring from 7 days. The bleachedspecimens had greater HO-1 immunolabelling in themiddle and cervical thirds of the coronal pulp at 2and 3 days, in all thirds at 7 days, and in the occlu-sal third at 15 days (P<0.05), and significantnuclear Jun-D immunolabelling in the cervical thirdat 2 and 3 days and in the occlusal and middle thirdsat 7 days (P<0.05). Bleached and control groupshad low numbers of CD90+/CD73+/CD105+/CD45 cells in the pulp at all periods (P>0.05).ConclusionsPulp cells responded to oxidative stressby expressing HO-1 during the post-bleaching inflam-mation phase until the beginning of the repair phase.Jun-D expression occurred during the reduction ofinflammation and the beginning of tertiary dentine production. The presence of oxidative stress did notinfluence the number of CD90+/CD73+/CD105+/CD45 cells identifiedin vivo in the dental pulppt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofInternational Endodontic Journalpt_BR
dc.rightsAcesso Restritopt_BR
dc.subjectDental pulppt_BR
dc.subjectDental bleachingpt_BR
dc.subjectHemeoxygenase-1pt_BR
dc.subjecthydrogen peroxidept_BR
dc.subjectJun-Dpt_BR
dc.subjectStem cellspt_BR
dc.subject.otherDental pulp cavitypt_BR
dc.subject.otherTooth bleachingpt_BR
dc.subject.otherHeme oxygenase-1pt_BR
dc.subject.otherHydrogen peroxidept_BR
dc.subject.otherStem cellspt_BR
dc.titleIn vivo analysis of the presence of heme oxygenase-1, transcription factor Jun-D and CD90+/CD73+/CD105+/CD45- cells in the pulp of bleached teethpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://onlinelibrary.wiley.com/doi/full/10.1111/iej.13190pt_BR
Appears in Collections:Artigo de Periódico

Files in This Item:
There are no files associated with this item.
Show simple item record


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.