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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/45699
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dc.creatorFrancine Benettipt_BR
dc.creatorAndré Luiz Fraga Brisopt_BR
dc.creatorMarina Carminattipt_BR
dc.creatorde Araújo Lopespt_BR
dc.creatorBarbosapt_BR
dc.creatorEdilson Ervolinopt_BR
dc.creatorJoão Eduardo Gomes Filhopt_BR
dc.creatorLuciano Tavares Angelo Cintrapt_BR
dc.date.accessioned2022-09-28T23:08:23Z-
dc.date.available2022-09-28T23:08:23Z-
dc.date.issued2019-
dc.citation.volume52pt_BR
dc.citation.issue05pt_BR
dc.citation.spage665pt_BR
dc.citation.epage675pt_BR
dc.identifier.doihttps://doi.org/10.1111/iej.13049pt_BR
dc.identifier.issn01432885pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/45699-
dc.description.resumoAim: To analyse the influence of H2 O2 on pulp repair through osteocalcin and osteopontin immunolabelling and in cellular defence by using the antireactive oxygen species (ROS) antibody. Methodology: The maxillary molars of 50 rats were treated with 35% H2 O2 (Ble groups) or placebo gel (control groups). At 0 h and 2, 7, 15 and 30 days (n = 10 hemimaxillae), the rats were killed and pulp tissue was evaluated using inflammation and immunolabelling scores (osteocalcin/osteopontin); ROS-positive cells were counted. Paired t-test and Wilcoxon signed-rank test were used (P < 0.05). Results: The Ble group had necrosis in the coronal pulp at 0 h and in the occlusal third of the coronal pulp at 2 days; at 7, 15 and 30 days, no inflammation was noted similar to the controls (P > 0.05). Osteocalcin was absent in the Ble at 0 h, moderate at 2 days and increased thereafter, differing from the controls at all two periods (P < 0.05). Osteopontin was higher principally at 7 and 15 days in Ble groups, but differing with control groups from 2 days after bleaching (P < 0.05). The Ble group had more ROS-positive cells in the pulp at 7 and 15 days (P < 0.05). Tertiary dentine was observed at 7 days, increasing thereafter (P < 0.05). Conclusions: Post-bleaching pulp repair was associated with increased osteocalcin over time. Osteopontin also participated in this process, and anti-ROS was involved in cellular defence against H2 O2 .pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofInternational Endodontic Journalpt_BR
dc.rightsAcesso Restritopt_BR
dc.subjectDental pulppt_BR
dc.subjectHydrogen peroxidept_BR
dc.subjectOsteocalcinpt_BR
dc.subjectOsteopontinpt_BR
dc.subjectReactive oxygen speciespt_BR
dc.subjectTertiary dentinept_BR
dc.subject.otherHydrogen peroxidept_BR
dc.subject.otherOsteocalcinpt_BR
dc.subject.otherOsteopontinpt_BR
dc.subject.otherAntibodiespt_BR
dc.subject.otherInflammationpt_BR
dc.titlePresence of osteocalcin, osteopontin, and reactive oxygen species-positive cells in pulp tissue after dental bleachingpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/iej.13049pt_BR
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