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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/46093
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dc.creatorIzabela Cristina Pires Gomespt_BR
dc.creatorAmanda Dayane Malta Matospt_BR
dc.creatorSilvia Nietschept_BR
dc.creatorAdelica Aparecida Xavierpt_BR
dc.creatorMárcia Regina Costapt_BR
dc.creatorWellington Silva Gomespt_BR
dc.creatorMarlon Cristian Toledo Pereirapt_BR
dc.date.accessioned2022-10-07T15:22:07Z-
dc.date.available2022-10-07T15:22:07Z-
dc.date.issued2017-
dc.citation.volume60pt_BR
dc.citation.spagee17160484pt_BR
dc.identifier.doi10.1590/1678-4324-2017160484pt_BR
dc.identifier.issn1678-4324pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/46093-
dc.description.resumoThis study aimed to evaluate the capacity for in vitro indole compound synthesis by endophytic bacteria and tocompare the Salkowski colorimetric and high-performance liquid chromatography (HPLC) quantification methods. Initial screenings were performed using 10% tryptic soy agar (TSA) medium with and without L-tryptophan via theSalkowski colorimetric method in a completely randomized design with 40 treatments and three replications. In thesecond experiment, the five most promising isolates were used for the detection and quantification of indoles by HPLC in a completely randomized design with five treatments and three replications. Of the 40 isolates, 16synthesized indole-3-acetic acid (IAA) in the presence of L-tryptophan, and only three synthesized IAA in theabsence of this amino acid. The concentration of IAA determined by absorbance indicated that Agrobacteriumtumefaciens isolate EB.07 showed a higher concentration of IAA in the presence of L-tryptophan (16.61 μ g mL -1 ). HPLC analysis revealed the production of the following four indole compounds by these bacteria: IAA, indole pyruvic acid (IPA), indole lactic acid (ILA), and indole-3-acetaldehyde (IAAld). Significant differences between thetwo estimation methods were observed for two of the five tested isolates: A. tumefaciens (EB.07) and Bacillus pumilus (EB.12). For both isolates, the Salkowski method indicated higher concentrations than those obtained with HPLC. Considering the obtained results, the Salkowski colorimetric method should be used in initial screenings,and HPLC should be used as a complementary method in the selection of isolates with the highest potential tosynthesize indoles.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIASpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofBrazilian Archives of Biology and Technologypt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectAIApt_BR
dc.subjectBacillus spp.pt_BR
dc.subjectHPLCpt_BR
dc.subjectcolorimetriapt_BR
dc.subject.otherPlantas - Efeito da auxinapt_BR
dc.subject.otherAlcalóides indólicospt_BR
dc.titleAuxin production by endophytic bacteria isolated from banana treespt_BR
dc.typeArtigo de Periódicopt_BR
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