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http://hdl.handle.net/1843/46489| Type: | Artigo de Periódico |
| Title: | The apical root canal system microbial communities determined by next-generation sequencing |
| Authors: | Luciana Carla Nevesde Brito Janet Doolittle-Hall Chun-Teh Lee Kevin Moss Wilson Bambirra Júnior Warley Luciano Fonseca Tavares Antônio Paulino Ribeiro Sobrinho Flávia Rocha Fonseca Teles |
| Abstract: | The aim of this study was to explore the microbial communities of endodontic infections at their apical portion by 16S rRNA Illumina sequencing and delineate the core microbiome of root canal infections and that of their associated clinical symptomatology. Samples were collected from fifteen subjects presenting one tooth with a root canal infection, and their associated symptoms were recorded. Samples were collected from the apical third of roots using a #10 K file and then amplified using multiple displacement amplification and PCR-amplified with universal primers. Amplicons were sequenced (V3-V4 hypervariable region of the 16S rRNA gene) using MiSeq (Illumina, CA). The microbial composition of the samples was determined using QIIME and HOMINGS. Data were analyzed using t tests and ANOVA. A total of 1,038,656 good quality sequences were obtained, and OTUs were assigned to 10 bacterial phyla, led by Bacteroidetes (51.2%) and Firmicutes (27.1%), and 94 genera were represented primarily by Prevotella (17.9%) and Bacteroidaceae G-1 (14.3%). Symptomatic teeth were associated with higher levels of Porphyromonas (p < 0.05) and Prevotella. P. endodontalis and P. oris were present in both cores. The present study demonstrated the complexity of the root canal microbiome and the "common denominators" of root canal infections and identified taxa whose virulence properties should be further explored. The polymicrobial etiology of endodontic infections has long been established. However, few studies have focused on expanding the breadth and depth of coverage of microbiome-infected root canals at their apical portion |
| Subject: | Infections Dental pulp cavity Endodontics RNA ribosomal 16s Host microbial interactions |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.1038/s41598-020-67828-3 |
| URI: | http://hdl.handle.net/1843/46489 |
| Issue Date: | 2-Jul-2020 |
| metadata.dc.url.externa: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331743/ |
| metadata.dc.relation.ispartof: | Scientific Reports |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| The apical root canal system microbial communities determined by next generation sequencing.pdf | 2.2 MB | Adobe PDF | View/Open |
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