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http://hdl.handle.net/1843/47968| Type: | Artigo de Periódico |
| Title: | Rapid microbiological tests for prospecting new fungal strains with high potentiality for the pectinolytic enzymes production |
| Authors: | Alex Sander Rodrigues Cangussu Mellanie Karoline do Carmo Félix Sérgio Carneiro Araújo Kelvinson Fernandes Viana Raimundo Wagner Souza Aguiar Berghem Morais Ribeiro Augustus Caeser Franke Portella Luiz Carlos Bertucci Barbosa Igor Viana Brandi Edson Wagner Silva Cangussu |
| Abstract: | Prospecting of new isolates producers of pectinolitic enzymes were performed with cultivation of substrates of rice, soybean and corn bran. Pectinolitic enzymes were observed in cultures of the isolates NFC 1, NFC 2, NFC 4, NFC 5 and NFC 6 identified as belonging to the genus Aspergillus sp. Isolates NFR 1, NFR 2 and NFS1 were identified as belonging to the genus Rhizopus and NFC 3 belonging to the genus Cladosporium; however, pectinolitic enzymes production was not observed. The polygalacturonase (PG) production in submerged fermentation of Aspergillus NFC 2 in culture medium proposed (SM) at 28 and 37°C was quantified by agar diffusion and spectrophotometric methods. The highest PG production was obtained at 28°C by greater formation of halo degradation pectin and the activity measured by spectrophotometric methods. The activity of PG obtained was 7 U.mL-1 with volumetric productivity greater in the first 24 h of fermentation both at 28 and 37°C. A decrease in PG synthesis occurred after 48 to 120 h. The study allowed, by means of rapid microbiological tests, the selection of new isolates fungi for PG production and the definition of crucial stages for cultivation in liquid medium. |
| Subject: | Fungos filamentosos Aspergillus Pectina Pectinase Fermentação |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.5897/AJMR2016.8236 |
| URI: | http://hdl.handle.net/1843/47968 |
| Issue Date: | 28-Sep-2016 |
| metadata.dc.url.externa: | https://academicjournals.org/journal/AJMR/article-abstract/8449CDE60748 |
| metadata.dc.relation.ispartof: | African Journal of Microbiology Research |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Rapid microbiological tests for prospecting new fungal strains with high potentiality for the pectinolytic enzymes production.pdf | 921.8 kB | Adobe PDF | View/Open |
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