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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/48132
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dc.creatorSahar Ansaript_BR
dc.creatorIvana Márcia Alves Dinizpt_BR
dc.creatorChider Chenpt_BR
dc.creatorTara Aghaloopt_BR
dc.creatorSongtao Shipt_BR
dc.creatorBenjamin Wupt_BR
dc.creatorAlireza Moshaveriniapt_BR
dc.date.accessioned2022-12-16T14:34:11Z-
dc.date.available2022-12-16T14:34:11Z-
dc.date.issued2017-09-15-
dc.citation.volume28pt_BR
dc.citation.issue10pt_BR
dc.citation.spage162pt_BR
dc.identifier.doi10.1007/s10856-017-5974-8pt_BR
dc.identifier.issn09574530pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/48132-
dc.description.resumoRepair or regeneration of damaged nerves is still a challenging clinical task in reconstructive surgeries and regenerative medicine. Here, it is demonstrated that periodontal ligament stem cells (PDLSCs) and gingival mesenchymal stem cells (GMSCs) isolated from adult human periodontal and gingival tissues assume neuronal phenotype in vitro and in vivo via a subcutaneous transplantation model in nude mice. PDLSCs and GMSCs are encapsulated in a 3D scaffold based on alginate and hyaluronic acid hydrogels capable of sustained release of human nerve growth factor (NGF). The elasticity of the hydrogels affects the proliferation and differentiation of encapsulated MSCs within scaffolds. Moreover, it is observed that PDLSCs and GMSCs are stained positive for βIII-tubulin, while exhibiting high levels of gene expression related to neurogenic differentiation (βIII-tubulin and glial fibrillary acidic protein) via quantitative polymerase chain reaction (qPCR). Western blot analysis shows the importance of elasticity of the matrix and the presence of NGF in the neurogenic differentiation of encapsulated MSCs. In vivo, immunofluorescence staining for neurogenic specific protein markers confirms islands of dense positively stained structures inside transplanted hydrogels. As far as it is known, this study is the first demonstration of the application of PDLSCs and GMSCs as promising cell therapy candidates for nerve regenerationpt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Materials Science-Materials in Medicinept_BR
dc.rightsAcesso Restritopt_BR
dc.subject.otherStem cellspt_BR
dc.subject.otherHyaluronic acidpt_BR
dc.subject.otherAlginatespt_BR
dc.subject.otherGingivapt_BR
dc.subject.otherPeriodonticspt_BR
dc.titleAlginate/hyaluronic acid hydrogel delivery system characteristics regulate the differentiation of periodontal ligament stem cells toward chondrogenic lineagept_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://link.springer.com/article/10.1007/s10856-017-5974-8pt_BR
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