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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/48139
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dc.creatorSahar Ansaript_BR
dc.creatorIvana Márcia Alves Dinizpt_BR
dc.creatorPatrícia Sarrionpt_BR
dc.creatorChider Chenpt_BR
dc.creatorAli Tamayolpt_BR
dc.creatorWupt_BR
dc.creatorAlireza Moshaveriniapt_BR
dc.date.accessioned2022-12-16T14:57:03Z-
dc.date.available2022-12-16T14:57:03Z-
dc.date.issued2017-
dc.citation.volume2017pt_BR
dc.citation.spage2017pt_BR
dc.identifier.doi10.1002/adhm.201700670pt_BR
dc.identifier.issn21922640pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/48139-
dc.description.resumoRepair or regeneration of damaged nerves is still a challenging clinical task in reconstructive surgeries and regenerative medicine. Here, we demonstrate that periodontal ligament stem cells (PDLSCs) and gingival mesenchymal stem cells (GMSCs) isolated from adult human periodontal and gingival tissues assume neuronal phenotype in vitro and in vivo via a subcutaneous transplantation model in nude mice. PDLSCs and GMSCs were encapsulated in a three dimensional scaffold based on alginate and hyaluronic acid hydrogels capable of sustained release of human nerve growth factor (NGF). We demonstrate that, the elasticity of the hydrogels affected the proliferation and differentiation of encapsulated MSCs within scaffolds. Moreover, we observed that PDLSCs and GMSCs were stained positive for βIII-tubulin, while exhibiting high levels of gene expression related to neurogenic differentiation (βIII-tubulin and GFAP) via qPCR. Western blot analysis showed the importance of the elasticity of the matrix and the presence of NGF in the neurogenic differentiation of encapsulated MSCs. In vivo, immunofluorescence staining for neurogenic specific protein markers confirmed islands of dense positively stained structures inside transplanted hydrogels. To our knowledge, this study is the first demonstration of the application of PDLSCs and GMSCs as promising cell therapy candidates for nerve regenerationpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofAdvanced Healthcare Materialspt_BR
dc.rightsAcesso Abertopt_BR
dc.subject.otherHydrogelspt_BR
dc.subject.otherHyaluronic acidpt_BR
dc.subject.otherGingivapt_BR
dc.subject.otherPeriodonticspt_BR
dc.subject.otherMesenchymal stem cellspt_BR
dc.titleHuman periodontal ligament- and gingiva-derived mesenchymal stem cells promote nerve regeneration when encapsulated in alginate/hyaluronic acid 3d scaffoldpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813692/pt_BR
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