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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/48155
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dc.creatorSandhra Maria de Carvalhopt_BR
dc.creatorAlice Gameiro Leonelpt_BR
dc.creatorAlexandra Ancelmo Piscitelli Mansurpt_BR
dc.creatorIsadora Cota Carvalhopt_BR
dc.creatorKlaus Wilhelm Heinrich Krambrockpt_BR
dc.creatorHerman Sander Mansurpt_BR
dc.date.accessioned2022-12-16T16:52:32Z-
dc.date.available2022-12-16T16:52:32Z-
dc.date.issued2019-
dc.citation.volume7pt_BR
dc.citation.issue5pt_BR
dc.citation.spage2102pt_BR
dc.citation.epage2122pt_BR
dc.identifier.doihttps://doi.org/10.1039/c8bm01528gpt_BR
dc.identifier.issn20474849pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/48155-
dc.description.resumoGlioblastoma is the most aggressive primary brain cancer, which has no cure yet. Emerging nanotheranostic alternatives such as magnetic iron oxide nanoparticles (MIONs) have great potential as multimodal cancer therapy mediators. They can act as nanocarriers of anticancer drugs and generate localized heat when exposed to an alternating magnetic field (AMF), resulting in combined effects of chemotherapy and magnetic hyperthermia therapy. Thus, we designed and synthesized novel MIONs directly through a co-precipitation method by a single step one-pot aqueous green process using carboxymethylcellulose (CMC) as a multifunctional, biocompatible and water-soluble biopolymer ligand (iron oxide nanoparticle-CMC, MION@CMC). They were bioconjugated via amide bonds with doxorubicin (DOX, an anticancer drug) forming nanohybrids (MION@CMC-DOX). The CMC, MION@CMC and MION@CMC-DOX nanoconjugates were comprehensively characterized by 1HNMR, FTIR, TEM/SAED/EDX, UV-visible, XRD, zeta potential (ZP) and DLS analyses. Moreover, cytotoxicity and cell killing activities of these nanoconjugates were assessed by in vitro biological assays. The nanoconjugates were incubated with glioma cells (U87), a magnetic hyperthermia (MHT) assay was performed for evaluating the activity against brain cancer cells and confocal laser scanning laser microscopy was used for bioimaging their cellular uptake pathways. The results showed that fairly monodisperse and water-soluble ultra-small iron oxide nanoparticles (Fe3O4) were synthesized (core size = 7 ± 2 nm) and stabilized by CMC producing negatively charged nanocolloids (-38 ± 3 mV, MION@CMC; hydrodynamic radius, HD = 38 ± 2 nm). The results confirmed the conjugation of MION@CMC with DOX by amide bonds, leading to the development of magnetopolymersome nanostructures (MION@CMC-DOX). The cell viability bioassays evidenced low toxicity of MION@CMC compared to the severe cytotoxicity of MION@CMC-DOX nanosystems mainly caused by the release of DOX. Under an alternating magnetic field, MION@CMC and MION@CMC-DOX systems demonstrated activity for killing U87 cancer cells due to the heat generated by hyperthermia. In addition, the MION@CMC-DOX bioconjugates showed significantly higher cell killing response when exposed to an AMF due to the combined chemotherapy effect of DOX release inside the cancer cells triggering apoptotic pathways.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorshipFINEP - Financiadora de Estudos e Projetos, Financiadora de Estudos e Projetospt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentENG - DEPARTAMENTO DE ENGENHARIA METALÚRGICApt_BR
dc.publisher.departmentICX - DEPARTAMENTO DE FÍSICApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofBiomaterials Sciencept_BR
dc.rightsAcesso Restritopt_BR
dc.subjectAntineoplastic agentspt_BR
dc.subjectBiological transportpt_BR
dc.subjectNanoparticlespt_BR
dc.subjectFerrosoferric oxidept_BR
dc.subject.otherAgentes antineoplásicospt_BR
dc.subject.otherTransporte biológicopt_BR
dc.subject.otherNanopartículaspt_BR
dc.subject.otherÓxido de ferropt_BR
dc.titleBifunctional magnetopolymersomes of iron oxide nanoparticles and carboxymethylcellulose conjugated with doxorubicin for hyperthermo-chemotherapy of brain cancer cellspt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://pubs.rsc.org/en/content/articlelanding/2019/BM/C8BM01528Gpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-8798-4182pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-1526-2508pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-2929-3452pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-7562-0285pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-3032-495Xpt_BR
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