1. Repositório Institucional da UFMG
  2. Publicações Científicas e Culturais
  3. Artigo de Periódico
Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/48171
Full metadata record
DC FieldValueLanguage
dc.creatorLeila Soares Ferreirapt_BR
dc.creatorIvana Márcia Alves Dinizpt_BR
dc.creatorCarlos Marandubapt_BR
dc.creatorSueli Patrícia Harumi Miyiagipt_BR
dc.creatorMaria Fernanda Rodriguespt_BR
dc.creatorCácio Moura-Netopt_BR
dc.creatorMárcia Martins Marquespt_BR
dc.date.accessioned2022-12-18T00:49:49Z-
dc.date.available2022-12-18T00:49:49Z-
dc.date.issued2018-09-24-
dc.citation.volume34pt_BR
dc.citation.spage659pt_BR
dc.citation.epage666pt_BR
dc.identifier.doihttps://doi.org/10.1007/s10103-018-2637-zpt_BR
dc.identifier.issn1435-604Xpt_BR
dc.identifier.urihttp://hdl.handle.net/1843/48171-
dc.description.resumoThe aim of this in vitro study was to analyze the effect of photobiomodulation therapy (PBMT) on the proliferation and undifferentiating status of stem cell from human exfoliated deciduous teeth (SHEDs). PBMT was carried out with an aluminum gallium indium phosphide (InGaAlP) diode laser in contact and punctual mode (continuous wave, 660 nm, 20 mW, 0.028 cm2, and average energy densities of 1 (1 s), 3 (4 s), 5 (7 s), 10 (14 s), 15 (21 s), or 20 (28 s) J/cm2 per point). The immunoprofile of the SHEDs was analyzed using flow cytometry. Cell proliferation was assessed by the MTT reduction assay. Gene expressions of mesenchymal stem cell markers (OCT4, Nestin, CD90, and CD105) were assessed by RT-qPCR 48 h after PBMT. Data were compared by analysis of variance (ANOVA) and Tukey's test (p ≤ 0.05). Cells cultured under nutritional deficit and treated with PBMT at 5 J/cm2 presented similar cell growth than those of positive control group. Cell growth was significantly higher than those of other groups. Mesenchymal stem cell gene markers were still expressed after PBMT at 5 J/cm2. In a short-term analysis, PBMT increases the number of stem cells with no interference in the undifferentiated state of the irradiated cells, which opens wide possibilities for application in tissue regeneration.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofLasers in Medical Sciencept_BR
dc.rightsAcesso Restritopt_BR
dc.subjectCell proliferationpt_BR
dc.subject.otherLasers semiconductorpt_BR
dc.subject.otherGene expressionpt_BR
dc.subject.otherMesenchymal stem cellspt_BR
dc.subject.otherCell proliferationpt_BR
dc.titleShort-term evaluation of photobiomodulation therapy on the proliferation and undifferentiated status of dental pulp stem cellspt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://link.springer.com/article/10.1007/s10103-018-2637-zpt_BR
Appears in Collections:Artigo de Periódico

Files in This Item:
There are no files associated with this item.
Show simple item record


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.