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Type:	Artigo de Periódico
Title:	Selection of resistance sources to common bean anthracnose by field phenotyping and DNA marker-assisted screening
Authors:	Ariadna Faria Vieira Demerson Arruda Sanglard Thiago Lívio Pessoa Oliveira de Souza Helton Santos Pereira Joaquim Geraldo Caprio da Costa Laura Cristina da Silva Almeida Luana Alves Rodrigues Leonardo Cunha Melo
Abstract:	The main goal of this work was to select resistance sources to common bean anthracnose by field phenotyping and DNA marker-assisted screening. Fifty-five common bean genotypes, including differential varieties, characterized resistance sources, elite lines, cultivars and controls, were evaluated in a field inoculation trial and screened with SCAR markers linked to resistance genes that are important in Brazil. The field trial was carried out in Santo Antônio de Goiás, GO, Brazil, during the fall/winter growing season of 2014, using artificial inoculation with a mixture of six races of Colletotrichum lindemuthianum, selected based on their high virulence and prevalence in Brazil. Amplification reactions with the SCAR markers previously identified as linked to important anthracnose resistance genes on Brazil followed standard procedures. Twenty-eight of the 58 genotypes were resistant to anthracnose (mean severity score ≤ 3.5). Ten of these 28 resistant genotypes stood out because they presented a mean anthracnose severity score of 1.0. Four of the six SCAR markers tested shown to be useful for the assisted selection of their respective target genes (SH18 and SAS13 for Co-42, SAB03 for Co-5, and SAZ20 for Co-6). Two carioca seeded elite lines were highlighted by the phenotypic and molecular screening: K10 (Co-34, Co-42, Co-5 and Co-6) and K13 (Co-4²). The phenotypic and molecular characterization of candidate resistance sources to common bean anthracnose based on their disease reaction in field inoculation trials and on the analysis with molecular markers linked to resistance genes has shown to be a useful strategy. These results aid in the selection of donor parents and resistant lines to be preferably explored by common bean breeding programs in Brazil.
Subject:	Fungos na agricultura Feijão Antracnose Genética molecular DNA Plantas -- Melhoramento genético
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
Rights:	Acesso Aberto
metadata.dc.identifier.doi:	https://doi.org/10.4238/gmr18066
URI:	http://hdl.handle.net/1843/48182
Issue Date:	2018
metadata.dc.url.externa:	https://geneticsmr.com/articles/selection-resistance-sources-common-bean
metadata.dc.relation.ispartof:	Genetics and Molecular Research
Appears in Collections:	Artigo de Periódico

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