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http://hdl.handle.net/1843/48525| Type: | Artigo de Periódico |
| Title: | Amino acid biosignature in plasma among ischemic stroke subtypes |
| Authors: | Vânia Aparecida Mendes Goulart Marcelo Martins de Sena Thiago de Oliveira Mendes Helvécio Costa Menezes Zenilda de Lourdes Cardeal Maria José Nunes de Paiva Valéria Cristina Sandrim Mauro Cunha Xavier Pinto Rodrigo Ribeiro Resende |
| Abstract: | Ischemic stroke is a neurovascular disorder caused by reduced or blockage of blood flow to the brain, which may permanently affect motor and cognitive abilities. The diagnostic of stroke is performed using imaging technologies, clinical evaluation, and neuropsychological protocols, but no blood test is available yet. In this work, we analyzed amino acid concentrations in blood plasma from poststroke patients in order to identify differences that could characterize the stroke etiology. Plasma concentrations of sixteen amino acids from patients with chronic ischemic stroke (n = 73) and the control group (n = 16) were determined using gas chromatography coupled to mass spectrometry (GC-MS). The concentration data was processed by Partial Least Squares-Discriminant Analysis (PLS-DA) to classify patients with stroke and control. The amino acid analysis generated a first model able to discriminate ischemic stroke patients from control group. Proline was the most important amino acid for classification of the stroke samples in PLS-DA, followed by lysine, phenylalanine, leucine, and glycine, and while higher levels of methionine and alanine were mostly related to the control samples. The second model was able to discriminate the stroke subtypes like atherothrombotic etiology from cardioembolic and lacunar etiologies, with lysine, leucine, and cysteine plasmatic concentrations being the most important metabolites. Our results suggest an amino acid biosignature for patients with chronic stroke in plasma samples, which can be helpful in diagnosis, prognosis, and therapeutics of these patients. |
| Subject: | Bioquímica Aminoácidos Marcadores biológicos Acidentes vasculares cerebrais Isquemia cerebral Espectrometria de massa |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA ICX - DEPARTAMENTO DE QUÍMICA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.1155/2019/8480468 |
| URI: | http://hdl.handle.net/1843/48525 |
| Issue Date: | 2019 |
| metadata.dc.url.externa: | https://www.hindawi.com/journals/bmri/2019/8480468/ |
| metadata.dc.relation.ispartof: | BioMed Research International |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Amino acid biosignature in plasma among ischemic stroke subtypes.pdf | 2.52 MB | Adobe PDF | View/Open |
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