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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/50584
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dc.creatorFlávia Oliveira Abrãopt_BR
dc.creatorEduardo Robson Duartept_BR
dc.creatorMoisés Sena Pessoapt_BR
dc.creatorVera Lúcia dos Santospt_BR
dc.creatorLuiz Fernando de Freitas Júniorpt_BR
dc.creatorKatharina de Oliveira Barrospt_BR
dc.creatorAlice Ferreira da Silva Hughespt_BR
dc.creatorThiago Dias Silvapt_BR
dc.creatorNorberto Mário Rodriguezpt_BR
dc.date.accessioned2023-03-02T11:15:16Z-
dc.date.available2023-03-02T11:15:16Z-
dc.date.issued2017-08-29-
dc.citation.volume12pt_BR
dc.citation.issue8pt_BR
dc.citation.spagee0183628pt_BR
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0183628pt_BR
dc.identifier.issn1932-6203pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/50584-
dc.description.resumoFungi have the ability to degrade vegetal cell wall carbohydrates, and their presence in the digestive tract of ruminants can minimize the effects of lignified forage on ruminal fermentation. Here, we evaluated enzyme production by Aspergillus spp. isolates from the digestive tracts of cattle grazed in tropical pastures during the dry season. Filamentous fungi were isolated from rumen and feces by culture in cellulose-based medium. Ninety fungal strains were isolated and identified by rDNA sequence analysis, microculture, or both. Aspergillus terreus was the most frequently isolated species, followed by Aspergillus fumigatus. The isolates were characterized with respect to their cellulolytic, xylanolytic, and lignolytic activity through qualitative evaluation in culture medium containing a specific corresponding carbon source. Carboxymethyl cellulase (CMCase) activity was quantified by the reducing sugar method. In the avicel and xilan degradation test, the enzyme activity (EA) at 48 h was significantly higher other periods (P < 0.05). Intra- and inter-specific differences in EA were verified, and high levels of phenoloxidases, which are crucial for lignin degradation, were observed in 28.9% of the isolates. Aspergillus terreus showed significantly higher EA for avicelase (3.96 ±1.77) and xylanase (3.13 ±.091) than the other Aspergillus species at 48 h of incubation. Isolates AT13 and AF69 showed the highest CMCase specific activity (54.84 and 33.03 U mg-1 protein, respectively). Selected Aspergillus spp. isolates produced remarkable levels of enzymes involved in vegetal cell wall degradation, suggesting their potential as antimicrobial additives or probiotics in ruminant diets.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorshipOutra Agênciapt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIASpt_BR
dc.publisher.initialsUFMGpt_BR
dc.rightsAcesso Abertopt_BR
dc.subject.otherBovino de cortept_BR
dc.subject.otherRuminantes - Alimentação e raçõespt_BR
dc.subject.otherFungos filamentosospt_BR
dc.subject.otherAspergilluspt_BR
dc.subject.otherAspergillus fumigatuspt_BR
dc.subject.otherRúmen - Microbiologiapt_BR
dc.titleNotable fibrolytic enzyme production by Aspergillus spp. isolates from the gastrointestinal tract of beef cattle fed in lignified pasturespt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0183628pt_BR
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