Estabelecimento e multiplicação in vitro de Stryphnodendron adstringens (Mart.) Coville

Abstract

Stryphnodendron adstringens (Mart.) Coville, popularly known as barbatimão, is a native species of the Brazilian Cerrado. Due to its pharmacological properties occurs a predatory exploitation of the species, which can lead it to the list of endangered species. For this reason, researches on the propagation of barbatimão are so important for the elaboration of protocols, which will give subsistence to the production of seedlings. In this context, the present work aimed to evaluate the establishment and multiplication in vitro of Stryphnodendron adstringens (Mart.) Coville. The first experiment addressed in vitro germination in MS and WPM culture media at different concentrations, in order to evaluate the culture media for the species and study its response to the procedure. The percentage of germination was similar between the culture media and their nutritional concentrations. Seedlings in the MS culture medium showed greater shoot length, of diameter and chlorophyll compared to the WPM medium, which demonstrated greater root length compared to the MS medium. The second experiment was carried out in the multiplication phase of apical segments of seedlings germinated in vitro, comparing regulators BAP and ANA at concentrations 0; 2.0; 4.0 and; 6.0 mg.L-1., with the purpose of evaluating the viability of micropropagation of the species for the production of clonal seedlings. The result demonstrated differences between the number of shoots produced by the explants in each treatment, so that with the addition of BAP the shoots multiplied and grew, while with the addition of ANA the shoots multiplied at a lower rate compared to the BAP and stagnated, demonstrating how hormonal balance occurs in barbatimão. It is concluded that the use of growth regulators of the cytokinin category favor the in vitro multiplication for Stryphnodendron adstringens, and that regulators of the auxin category can be used for other stages of micropropagation, being expendable in the sprout multiplication process. It was concluded that for the establishment and multiplication in vitro of S. adstringens can be used MS medium at a concentration of 50%, with the addition of BAP at concentrations from 2.0 to 6.0 mg.L-1 for the multiplication phase.