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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/51013
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dc.creatorDanielle Campiol Arrudapt_BR
dc.creatorArmando da Silva Cunha Júniorpt_BR
dc.creatorÂngelo Malachias de Souzapt_BR
dc.creatorVirginie Escrioupt_BR
dc.creatorIsmael José Gonzalezpt_BR
dc.creatorStéphanie Finetpt_BR
dc.creatorLuis Cordovapt_BR
dc.creatorValérie Trichetpt_BR
dc.creatorGracielle Ferreira Andradept_BR
dc.creatorCéline Hoffmannpt_BR
dc.creatorPascal Bigeypt_BR
dc.creatorWaldemar Augusto de Almeida Macedopt_BR
dc.date.accessioned2023-03-17T19:21:11Z-
dc.date.available2023-03-17T19:21:11Z-
dc.date.issued2019-
dc.citation.volume540pt_BR
dc.citation.spage342pt_BR
dc.citation.epage353pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.jcis.2019.01.043pt_BR
dc.identifier.issn1095-7103pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/51013-
dc.description.resumoVectorized small interfering RNAs (siRNAs) are widely used to induce specific mRNA degradation in the intracellular compartment of eukaryotic cells. Recently, we developed efficient cationic lipid-based siRNA vectors (siRNA lipoplexes or siLex) containing sodium alginate (Nalg-siLex) with superior efficiency and stability properties than siLex. In this study, we assessed the physicochemical and some biological properties of Nalg-siLex compared to siLex. While no significant differences in size, ζ potential and siRNA compaction were detected, the addition of sodium alginate modified the particle morphology, producing smoother and heterogeneous particles characterized by transmission electron microscopy. We also noted that Nalg-siLex have surface differences observed by X-ray photoelectron spectroscopy. These differences could arise from an internal reorganization of components induced by the addition of sodium alginate, that is indicated by Small-Angle X-ray Scattering results. Moreover, Nalg-siLex did not trigger significant hepatotoxicity nor inflammatory cytokine secretion compared to siLex. Taken together these results suggest that sodium alginate played a key role by structuring and reinforcing siRNA lipoplexes, leading to more stable and efficient delivery vector.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOSpt_BR
dc.publisher.departmentICX - DEPARTAMENTO DE FÍSICApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Colloid and Interface Science-
dc.rightsAcesso Restritopt_BR
dc.subjectRNA interferencept_BR
dc.subjectCationic lipidpt_BR
dc.subjectNanoparticlept_BR
dc.subjectDelivery systempt_BR
dc.subjectAnionic polymerpt_BR
dc.subject.otherÁcido ribonucleicopt_BR
dc.subject.otherNanopartículaspt_BR
dc.titleModifying internal organization and surface morphology of siRNA lipoplexes by sodium alginate addition for efficient siRNA deliverypt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.sciencedirect.com/science/article/pii/S0021979719300542pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-9838-728Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-1161-8936pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-8703-4283pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-5686-9301pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-9036-2835pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-4183-9032pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-9038-1254pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-9658-6373pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-6795-8569pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-5451-9965pt_BR
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