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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/51111
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dc.creatorLavany Aajay Sharmapt_BR
dc.creatorRobert Lovept_BR
dc.creatorMohammad Alipt_BR
dc.creatorAjay Sharmapt_BR
dc.creatorSoraia Macaript_BR
dc.creatorAvadhoot Avadhanipt_BR
dc.creatorGeorge Diaspt_BR
dc.date.accessioned2023-03-22T00:40:59Z-
dc.date.available2023-03-22T00:40:59Z-
dc.date.issued2017-04-20-
dc.citation.volume1pt_BR
dc.citation.spage1pt_BR
dc.citation.epage7pt_BR
dc.identifier.doihttps://doi.org/10.5301/jabfm.5000346pt_BR
dc.identifier.issn22808000pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/51111-
dc.description.resumoBackground Keratin has shown promising outcomes as a biomaterial due to its inherent bioactivity, biocompatibility and regenerative effects. The effect of keratin on repair and regeneration of dental tissues has never been studied before. Current therapies to treat pulp tissues involve its replacement with inert, synthetic materials that do not have a proper biological function, leading to failure and tooth loss. This study aimed to develop a biocompatible keratin hydrogel (KH) suitable for pulp therapies. Methods Keratins extracted from sheep wool were isolated, quantified and reconstituted to form KH. Different concentrations of keratin gel suitable for dental application were characterized by rheological analysis. The optimized gel based on flow characteristics was studied further for microstructure including porosity, percentage swelling ratio and contact angle measurements, using analytical tools such as scanning electron microscopy (SEM), micro-computed tomography and goniometer. To assess both biocompatibility and pulpal response, KH was implanted into rat upper molar teeth following partial pulpotomy. After 28 days, the tissue sections were analyzed by histological and immunohistochemical methods to identify dentin matrix protein 1 (DMP-1) formation and compared with control (Ca(OH)2-treated) teeth. Results The results of the study demonstrated a viscous and injectable, porous, dimensionally stable, hydrophilic and biocompatible gel that allowed pulp healing to occur by a reparative response, with widespread DMP-1 expression. Conclusions The findings of this study indicate that keratins can be developed as a biomaterial source for alternate biological treatment options for pulp therapies.pt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Applied Biomaterials & Functional Materialspt_BR
dc.rightsAcesso Abertopt_BR
dc.subject.otherKeratinspt_BR
dc.subject.otherToothpt_BR
dc.subject.otherDental pulp cavitypt_BR
dc.subject.otherProteinspt_BR
dc.subject.otherBiocompatible materialspt_BR
dc.subject.otherDentinpt_BR
dc.subject.otherRegenerative medicinept_BR
dc.titleHealing response of rat pulp treated with an injectable keratin hydrogelpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://journals.sagepub.com/doi/10.5301/jabfm.5000346?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmedpt_BR
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