Simultaneous saccharification isomerization and co-fermentation - SSICF: a new process concept for second-generation ethanol biorefineries combining immobilized recombinant enzymes and non-GMO saccharomyces

Ederson Pauloxavier Guilherme; Leticia Maria Zanphorlin; Amanda Silva de Sousa; Renan Yuji Miyamoto; Carlos Giovani Oliveira Bruziquesi; Bruna Mara Aparecida de Carvalho Mesquita; Sergio Henrique Sousa Santos; Elizama Aguiar de Oliveira; Junio Cota

Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/52384

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DC Field	Value	Language
dc.creator	Ederson Pauloxavier Guilherme	pt_BR
dc.creator	Leticia Maria Zanphorlin	pt_BR
dc.creator	Amanda Silva de Sousa	pt_BR
dc.creator	Renan Yuji Miyamoto	pt_BR
dc.creator	Carlos Giovani Oliveira Bruziquesi	pt_BR
dc.creator	Bruna Mara Aparecida de Carvalho Mesquita	pt_BR
dc.creator	Sergio Henrique Sousa Santos	pt_BR
dc.creator	Elizama Aguiar de Oliveira	pt_BR
dc.creator	Junio Cota	pt_BR
dc.date.accessioned	2023-04-24T12:59:54Z	-
dc.date.available	2023-04-24T12:59:54Z	-
dc.date.issued	2021	-
dc.citation.volume	182	pt_BR
dc.citation.spage	274	pt_BR
dc.citation.epage	284	pt_BR
dc.identifier.doi	https://doi.org/10.1016/j.renene.2021.10.023	pt_BR
dc.identifier.issn	0960-1481	pt_BR
dc.identifier.uri	http://hdl.handle.net/1843/52384	-
dc.description.resumo	Integrated bioprocess strategies may facilitate ethanol production from both C6 and C5 fractions of lignocellulosic feedstocks. We propose a new process concept, SSICF, where sugarcane bagasse is hydrolyzed simultaneously with xylose isomerization and the co-fermentation of C6 and C5 sugars. A commercial cocktail was supplemented with our multi-enzymatic system composed of three recombinant enzymes immobilized in Feroxyhyte magnetic nanoparticles: β-glucosidase, β-xylosidase and xylose isomerase. SSICF was performed using non-GMO Saccharomyces at pH 6.0 and 35 °C for 72 h in a synthetic medium containing cellobiose and xylose, and another medium containing pretreated sugarcane bagasse (PSB). The results of ethanol global yields in SSICF were 77.67% and 73.24% for the synthetic medium and PSB, respectively. In a nutshell, this is the first report of a successful proof-of-concept of SSICF with four rounds of enzyme recycling and a non-GMO yeast, an innovative process with high potential for industrial use.	pt_BR
dc.description.sponsorship	CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico	pt_BR
dc.description.sponsorship	FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais	pt_BR
dc.description.sponsorship	CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior	pt_BR
dc.language	eng	pt_BR
dc.publisher	Universidade Federal de Minas Gerais	pt_BR
dc.publisher.country	Brasil	pt_BR
dc.publisher.department	ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS	pt_BR
dc.publisher.initials	UFMG	pt_BR
dc.relation.ispartof	Renewable Energy	-
dc.rights	Acesso Restrito	pt_BR
dc.subject.other	Óxidos	pt_BR
dc.subject.other	Hidróxidos	pt_BR
dc.subject.other	Inibidores enzimáticos	pt_BR
dc.subject.other	Biomassa	pt_BR
dc.subject.other	Fermentação	pt_BR
dc.subject.other	Álcool	pt_BR
dc.title	Simultaneous saccharification isomerization and co-fermentation - SSICF: a new process concept for second-generation ethanol biorefineries combining immobilized recombinant enzymes and non-GMO saccharomyces	pt_BR
dc.type	Artigo de Periódico	pt_BR
dc.url.externa	https://www.sciencedirect.com/science/article/pii/S0960148121014804	pt_BR
Appears in Collections:	Artigo de Periódico

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