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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/53795
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dc.creatorJanete Maria da Silva Alvespt_BR
dc.creatorPricila Julieta Dantas Rochapt_BR
dc.creatorEduardo Robson Duartept_BR
dc.creatorHigor Alexandre Rodrigues Maiapt_BR
dc.creatorCláudio Eduardo Silva Freitaspt_BR
dc.creatorMárcio Antônio Silva Pimentapt_BR
dc.creatorHenrique Maia Valériopt_BR
dc.date.accessioned2023-05-23T11:29:30Z-
dc.date.available2023-05-23T11:29:30Z-
dc.date.issued2021-09-
dc.citation.volume36pt_BR
dc.citation.spage102143pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.bcab.2021.102143pt_BR
dc.identifier.issn1878-8181pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/53795-
dc.description.resumoDegradation of lignocellulosic materials by Aspergillus terreus isolated from rumen fluid, fungal biomass, in vitro substrate degradation, pH at end of incubation, concentrations of total protein (TP) and total cellulase (TC) were performed.In prior assay, submerged fermentations of sugarcane bagasse (SB) or Urochloa decumbens (UD) by five A.terreus isolates were performed.Subsequently, submerged fermentation using the two most promising isolates were evaluated comparing two nitrogen sources for cellulases, CMCases, avicelase and xylanases productions at 0, 72, 144, 216 and up to 288 h, in a factorial (2 × 2 × 5). During fermentation with UD and SB, showing lignin values 7.50 and 6.15%, respectively, the enzymatic activities at 50 °C were higher than those observed in the pH and temperature simulating the rumen (39 °C), allowing us to determine the optimal conditions under which the enzymes of interest may function in the animal model.The interaction between substrates and the evaluated periods was significant. Specifically for UD, the isolates V15 and V22 produced 226.14 and 210.72 nmol/L.s, respectively, which concentrations did not differ from each other.The isolates of this fungus showed growth ranging from 669 mg/L for the V79 isolate, using UD, and o 19,695 mg/L for the V22 isolate using SB after 216 h in medium supplemented with ammonium sulfate.Total protein levels were influenced by the substrates evaluated and by fermentation periods. The activity carboxymethylcellulases (CMCases) and xylanases presented values between 15.42 and 92.51 nmol/L.s for SB and from 5.14 to 226.14 nmol/L.s for UD fermentations. Using urea or ammonium sulfate, the V79 isolate in SB fermentation and the V49 isolate in UD fermentation, at 288 h, presented the highest rates of TP. The quantifications of TC in pH 4.5 at 50 °C were higher presenting 12,380.97 nmol/L.s and PT 347.575,0 nmol/mg.s, respectively by biomass, than those produced at pH 6.8 and 39 °C. The two selected A. terreus isolates from bovine rumen showed potential to use in the industrial microbiology and as additives to diets of ruminants.pt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIASpt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofBiocatalysis and Agricultural Biotechnology-
dc.rightsAcesso Restritopt_BR
dc.subject.otherBovino de cortept_BR
dc.subject.otherRuminantes - Alimentação e raçõespt_BR
dc.subject.otherCapim-braquiáriapt_BR
dc.subject.otherBagaço de canapt_BR
dc.subject.otherHidrólisept_BR
dc.subject.otherRúmen - Microbiologiapt_BR
dc.titleEnzymatic profiles of hydrolysis of lignocellulosic materials from Aspergillus terreus strains isolated from the rumen of beef cattle from Brazilpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.sciencedirect.com/science/article/pii/S1878818121002395pt_BR
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