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http://hdl.handle.net/1843/53796| Tipo: | Artigo de Periódico |
| Título: | Hydrolysis of lignocellulosic forages by Trichoderma longibrachiatum isolate from bovine rumen |
| Autor(es): | Eduardo Robson Duarte Higor Alexandre Rodrigues Maia Cláudio Eduardo Silva Freitas Janete Maria da Silva Alves Henrique Maia Valério Júnio Cota Silva |
| Resumo: | Fungi of the genus Trichoderma hydrolyze lignocellulosic materials, thus promoting the production of fermentable sugars. In the present study, the production of cellulases by Trichoderma longibrachiatum from ruminal fluid was evaluated in submerged fermentation of Urochloa decumbens hay (UD) or sugarcane bagasse (SB). Moreover, the biomass of this fungus was analyzed by supplementing it with urea or ammonium sulfate and with two buffer systems for the production of cellulases by T. longibrachiatum using SB or UD. The fermentations were carried out in test tubes containing Sabouraud broth, and the carbon source was packed in nonwoven fabric sachets. The fermentations were performed at 39 °C for 10 days. The factorial design was completely randomized, and the final pH, fungal biomass, substrate dry matter degradation, total protein, and cellulolytic enzymes were evaluated after the fermentation process. The fungal development was better in the medium supplemented with ammonium sulfate than that supplemented with urea, and it showed better degradation of UB than SB. The evaluated isolate promoted better protein synthesis during fermentation of SB buffered with sodium acetate; however, the medium containing UB produced higher concentrations of total cellulase, carboxymethylcellulase, and avicelase than the medium containing SB. Thus, rapid cellulose production by this fungus may aid in the hydrolysis of lignocellulosic materials for animal feed supplementation for ruminants; furthermore, the use of these enzymes for biotechnological purposes is promising. |
| Assunto: | Bovino de corte Bovinos - Alimentação e rações Ruminantes - Alimentação e rações Nutrição animal Bagaço de cana Capim-braquiária Forragem Hidrólise Rúmen - Microbiologia Trichoderma |
| Idioma: | eng |
| País: | Brasil |
| Editor: | Universidade Federal de Minas Gerais |
| Sigla da Instituição: | UFMG |
| Departamento: | ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS ICB - DEPARTAMENTO DE MICROBIOLOGIA |
| Tipo de Acesso: | Acesso Restrito |
| Identificador DOI: | https://doi.org/10.1016/j.bcab.2021.102135 |
| URI: | http://hdl.handle.net/1843/53796 |
| Data do documento: | Set-2021 |
| metadata.dc.url.externa: | https://www.sciencedirect.com/science/article/pii/S1878818121002310 |
| metadata.dc.relation.ispartof: | Biocatalysis and Agricultural Biotechnology |
| Aparece nas coleções: | Artigo de Periódico |
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