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http://hdl.handle.net/1843/53961| Type: | Artigo de Periódico |
| Title: | Bovine milk extracellular vesicles are osteoprotective by increasing osteocyte numbers and targeting RANKL/OPG system in experimental models of bone loss |
| Authors: | Marina Chaves de Oliveira Bartijn Pieters Polianna B. Guimarães Letícia F. Duffles Joyce Elisa Heredia Ana Letícia Malheiros Silveira Amanda C. C. Oliveira Mauro Martins Teixeira Adaliene Versiani Matos Ferreira Tarcilia A Silva Fons Van De Loo Soraia Macari |
| Abstract: | Studying effects of milk components on bone may have a clinical impact as milk is highly associated with bone maintenance, and clinical studies provided controversial associations with dairy consumption. We aimed to evaluate the impact of milk extracellular vesicles (mEVs) on the dynamics of bone loss in mice. MEVs are nanoparticles containing proteins, mRNA and microRNA, and were supplemented into the drinking water of mice, either receiving diet-induced obesity or ovariectomy (OVX). Mice receiving mEVs were protected from the bone loss caused by diet-induced obesity. In a more severe model of bone loss, OVX, higher osteoclast numbers in the femur were found, which were lowered by mEV treatment. Additionally, the osteoclastogenic potential of bone marrow-derived precursor cells was lowered in mEV-treated mice. The reduced stiffness in the femur of OVX mice was consequently reversed by mEV treatment, accompanied by improvement in the bone microarchitecture. In general, the RANKL/OPG ratio increased systemically and locally in both models and was rescued by mEV treatment. The number of osteocytes, as primary regulators of the RANKL/OPG system, raised in the femur of the OVX mEVs-treated group compared to OVX nontreated mice. Also, the osteocyte cell line treated with mEVs demonstrated a lowered RANKL/OPG ratio. Thus, mEVs showed systemic and local osteoprotective properties in two mouse models of bone loss reflected in reduced osteoclast presence. Data reveal mEV potential in bone modulation, acting via osteocyte enhancement and RANKL/OPG regulation. We suggest that mEVs could be a therapeutic candidate for the treatment of bone loss. |
| Subject: | Vesículas extracelulares Leite Osteoporose Obesidade Osteócitos |
| language: | por |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ENF - DEPARTAMENTO DE NUTRIÇÃO FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA ICB - DEPARTAMENTO DE FARMACOLOGIA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.3389/fbioe.2020.00891 |
| URI: | http://hdl.handle.net/1843/53961 |
| Issue Date: | 2020 |
| metadata.dc.url.externa: | https://www.frontiersin.org/articles/10.3389/fbioe.2020.00891/full |
| metadata.dc.relation.ispartof: | Frontiers in bioengineering and biotechnology |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Bovine Milk Extracellular Vesicles Are Osteoprotective by Increasing Osteocyte Numbers and Targeting RANKL_OPG System in Experimental Models of Bone Loss.pdf | 25.54 MB | Adobe PDF | View/Open |
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