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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/54694
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dc.creatorLetícia da Conceição Bragapt_BR
dc.creatorAgnaldo Lopes da Silva Filhopt_BR
dc.creatorWarne Pedro Andradept_BR
dc.creatorRafaela de Souza Furtadopt_BR
dc.creatorLuciana Maria Silvapt_BR
dc.creatorNikole Gontijo Gonçalvespt_BR
dc.date.accessioned2023-06-08T00:10:38Z-
dc.date.available2023-06-08T00:10:38Z-
dc.date.issued2020-
dc.citation.volume75pt_BR
dc.identifier.doihttps://doi.org/10.6061/clinics/2020/e1492pt_BR
dc.identifier.issn1980-5322pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/54694-
dc.description.resumoOBJECTIVES: The objectives of this study were to determine the sensitivity of ovarian cancer (OC) cell lines (TOV-21G and SKOV-3) to cisplatin and to the recombinant human TRAIL (rhTRAIL), and to evaluate the expression profile of TNFRSF10B, TNFRSF10C, TP53TG5, MDM2, BAX, BCL-2 and CASPASE-8 genes and their participation in the resistance/susceptibility mechanism of these tumor cell lines. METHODS: To determine the IC50 values associated with Cisplatin and rhTRAIL, inhibition of cell growth was observed using MTT assays in two human OC cell lines (SKOV-3 and TOV-21G). The analysis of gene expression was performed using quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS: Both cell lines had different susceptibility profiles to the tested drugs. In the SKOV-3 cell line, the IC50 values for cisplatin and for rhTRAIL were 270.83 ug/mL and 196.5 ng/mL, respectively. The same concentrations were used for TOV-21G. Different gene expression profiles were observed in each tested cell line. CASPASE-8 and TNFRSF10B expression levels could predict the response of both the cell lines to rhTRAIL alone or the response to a combination of rhTRAIL and cisplatin. In addition, we observed a relationship between BCL-2 and BAX expression that may be helpful in estimating the proliferation rate of the OC cell lines. CONCLUSION: SKOV-3 and TOV-21G respond differently to cisplatin and rhTRAIL exposure, and expression of CASPASE-8 and TNFRSF10B are good predictors of responses to these treatments.pt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentMED - DEPARTAMENTO DE GINECOLOGIA OBSTETRÍCIApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofClinics-
dc.rightsAcesso Abertopt_BR
dc.subjectOvarian cancerpt_BR
dc.subjectGene expressionpt_BR
dc.subjectChemoresistancept_BR
dc.subjectApoptosispt_BR
dc.subjectTrailpt_BR
dc.subject.otherNeoplasias ovarianaspt_BR
dc.subject.otherExpressão gênicapt_BR
dc.subject.otherApoptosept_BR
dc.titleApoptosis-related gene expression can predict the response of ovarian cancer cell lines to treatment with recombinant human TRAIL alone or combined with cisplatinpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.scielo.br/j/clin/a/9ZGtfXVCX5w7K9wXRVhPYSK/?lang=enpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-8486-7861pt_BR
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