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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/55170
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dc.creatorFernanda Badottipt_BR
dc.creatorFranscislon Silva de Oliveirapt_BR
dc.creatorCleverson Fernando Garciapt_BR
dc.creatorAline Bruna Martins Vazpt_BR
dc.creatorPaula Luize Camargos Fonsecapt_BR
dc.creatorLaila Alves Nahumpt_BR
dc.creatorGuilherme Oliveirapt_BR
dc.creatorAristóteles Góes-Netopt_BR
dc.date.accessioned2023-06-20T19:26:47Z-
dc.date.available2023-06-20T19:26:47Z-
dc.date.issued2017-
dc.citation.volume17pt_BR
dc.citation.issue42pt_BR
dc.identifier.doihttp://dx.doi.org/10.1186/s12866-017-0958-xpt_BR
dc.identifier.issn1471-2180pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/55170-
dc.description.resumoBackground: Fungi are among the most abundant and diverse organisms on Earth. However, a substantial amount of the species diversity, relationships, habitats, and life strategies of these microorganisms remain to be discovered and characterized. One important factor hindering progress is the difficulty in correctly identifying fungi. Morphological and molecular characteristics have been applied in such tasks. Later, DNA barcoding has emerged as a new method for the rapid and reliable identification of species. The nrITS region is considered the universal barcode of Fungi, and the ITS1 and ITS2 sub-regions have been applied as metabarcoding markers. In this study, we performed a large-scale analysis of all the available Basidiomycota sequences from GenBank. We carried out a rigorous trimming of the initial dataset based in methodological principals of DNA Barcoding. Two different approaches (PCI and barcode gap) were used to determine the performance of the complete ITS region and sub-regions. Results: For most of the Basidiomycota genera, the three genomic markers performed similarly, i.e., when one was considered a good marker for the identification of a genus, the others were also; the same results were observed when the performance was insufficient. However, based on barcode gap analyses, we identified genomic markers that had a superior identification performance than the others and genomic markers that were not indicated for the identification of some genera. Notably, neither the complete ITS nor the sub-regions were useful in identifying 11 of the 113 Basidiomycota genera. The complex phylogenetic relationships and the presence of cryptic species in some genera are possible explanations of this limitation and are discussed. Conclusions: Knowledge regarding the efficiency and limitations of the barcode markers that are currently used for the identification of organisms is crucial because it benefits research in many areas. Our study provides information that may guide researchers in choosing the most suitable genomic markers for identifying Basidiomycota species.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipOutra Agênciapt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofBMC Microbiologypt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectITSpt_BR
dc.subjectITS1pt_BR
dc.subjectITS2pt_BR
dc.subjectProbable correct identificationpt_BR
dc.subjectBarcode gappt_BR
dc.subjectBasidiomycotapt_BR
dc.subject.otherDNApt_BR
dc.subject.otherCódigo de Barras de DNA Taxonômicopt_BR
dc.subject.otherBasidiomycotapt_BR
dc.titleEffectiveness of ITS and sub-regions as DNA barcode markers for the identification of Basidiomycota (Fungi)pt_BR
dc.title.alternativeEficácia de ITS e sub-regiões como marcadores de código de barras de DNA para a identificação de Basidiomycota (Fungos)pt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-017-0958-xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-9038-4631pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8593-052Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-9354-1401pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-3167-6308pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-7692-6243pt_BR
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