1. Repositório Institucional da UFMG
  2. Publicações Científicas e Culturais
  3. Artigo de Periódico
Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/55292
Full metadata record
DC FieldValueLanguage
dc.creatorLaura Néspoli Nassar Pansinide Jesuspt_BR
dc.creatorDaniel Vitor Vasconcelos-santospt_BR
dc.creatorJosé Nélio Januáriopt_BR
dc.creatorAndréa Teixeira-carvalhopt_BR
dc.creatorRicardo Wagner Almeida Vitorpt_BR
dc.creatorEloísa A.v. Ferropt_BR
dc.creatorJosé Roberto Mineopt_BR
dc.creatorLilian Maria Garcia Bahia-oliveirapt_BR
dc.creatorOlindo Assis Martins-filhopt_BR
dc.creatorElenice Moreira Lemospt_BR
dc.creatorAline de Castro Zacche Toninipt_BR
dc.creatorGeisa Baptista Barrospt_BR
dc.creatorJordana Grazziela a. Coelho-dos-reispt_BR
dc.creatorSamantha Ribeiro Bélapt_BR
dc.creatorLis Ribeiro do Valle Antonellipt_BR
dc.creatorAnderson Silva Machadopt_BR
dc.creatorAna Carolina de Aguiar Vasconcelos Carneiropt_BR
dc.creatorGláucia Manzan Queiroz Andradept_BR
dc.date.accessioned2023-06-23T20:29:57Z-
dc.date.available2023-06-23T20:29:57Z-
dc.date.issued2016-
dc.citation.volume428pt_BR
dc.citation.spage1pt_BR
dc.citation.epage8pt_BR
dc.identifier.doi10.1016/j.jim.2015.11.004pt_BR
dc.identifier.issn00221759pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/55292-
dc.description.resumoThis study intended to apply the flow cytometric analysis of IgA and IgG reactivity and intracytoplasmic cytokine analysis to understand and decode the clinical aspects of infants with ocular congenital toxoplasmosis. The Toxoplasma gondii-infected infants (TOXO) were subdivided according to their clinical aspects based on the absence (NRL), presence of active (ARL), active/cicatricial (ACRL) or cicatricial retinochoroidal lesions (CRL) and compared to non-infected controls (NI). The reactivity of anti-T. gondii IgG subclasses resembles the clinical aspects of ocular lesions. IgG and IgG1 discriminate infants with cicatricial lesions (ACRL and CRL) from both ARL and NLR. IgG2 and IgG3 are particularly higher in ACRL and CRL as compared to NLR. No differences were ob served when IgG4 reactivity was evaluated. Thus, the results indicated that the reactivity patterns of IgA, IgG and IgG subclasses are able to discriminate ARL, ACRL and CRL from NLR or NI. IgA and IgG subclasses are relevant serological biomarkers with diagnostic and prognostic applicability, respectively. Moreover, IgA and IgG1 wereclosely related to cytokine production by innate/adaptive immunity cells. IgA reactivity was directly associated to TNF-α-derived from neutrophils, monocytes and CD8+ T-cells, while IgG1 was inversely correlated with IFN-γ-producing CD4+ and CD8+ T-cells but positively correlated with IL-10+ B-cells. These findings provide insights on the relationship between the cytokine production by innate/adaptive immunity and the antibody pattern of infants with ocular congenital toxoplasmosis. In addition, the present study supports the use of flow cytometric serology as a potential tool for the diagnosis and monitoring of ocular lesions in T. gondii-infected infants in the clinical setting.pt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE PARASITOLOGIApt_BR
dc.publisher.departmentMED - DEPARTAMENTO DE CLÍNICA MÉDICApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Immunological Methods-
dc.rightsAcesso Restritopt_BR
dc.subjectToxoplasma gondiipt_BR
dc.subjectCongenital toxoplasmosispt_BR
dc.subjectIgApt_BR
dc.subjectIgG1pt_BR
dc.subject.otherToxoplasmapt_BR
dc.subject.otherToxoplasmose Congênitapt_BR
dc.subject.otherImunoglobulina Apt_BR
dc.subject.otherImunoglobulina Gpt_BR
dc.titleIgA and IgG1 reactivities assessed by flow cytometry mirror clinical aspects of infants with ocular congenital toxoplasmosispt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.sciencedirect.com/science/article/pii/S0022175915300600pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0912-3782pt_BR
Appears in Collections:Artigo de Periódico

Files in This Item:
There are no files associated with this item.
Show simple item record


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.