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http://hdl.handle.net/1843/55299| Type: | Artigo de Periódico |
| Title: | Establishing tools for early diagnosis of congenital toxoplasmosis: flow cytometric igg avidity assay as a confirmatory test for neonatal screening |
| Authors: | Alinede Castro Zacche-tonini Daniel Vítor de Vasconcelos Santos José Nélio Januário Andréa Teixeira-carvalho Ricardo Wagner Almeida Vitor Eloísa Amália Vieira Ferro José Roberto Mineo Olindo Assis Martins-filho Elenice Moreira Lemos Giuliana Schmidt França Fonseca Laura Néspoli Nassar Passini de Jesus Geisa Baptista Barros Jordana Grazziela Alves Coelho-dos-reis Samantha Ribeiro Béla Anderson Silva Machado Ana Carolina Aguiar Vasconcelos Carneiro Gláucia Manzan Queiroz Andrade |
| Abstract: | The aim of this study was to evaluate the performance of conventional serology (Q-Preven™ and ELFAVIDAS™)and flow cytometry-based serologic tools for early serologic diagnosis of congenital toxoplasmosis. The study groups included prospectively confirmed cases of congenital toxoplasmosis (TOXO = 88) and age-matching non infected controls (NI = 15).The results demonstrated that all samples tested positive/indeterminate for anti-T.gondii IgM screening at birth using air-dried whole blood samples. Serum samples collected at 30–45 days after birth tested positive for ELFAVIDAS™ IgG in both groups. While all NI tested negative for ELFAVIDAS™ IgM and IgA, only 78% and 36% of TOXO tested positive for IgM and IgA, respectively. Flow cytometry-based anti-T. gondii IgM, IgA and IgG reactivity displayed moderate performance with low sensitivity (47.6%, 72.6% and 75.0%, respectively). Regardless the remarkable specificity of IgG1, IgG2 and IgG3 subclasses for early diagnosis,weak or moderate specificity was observed (Se = 73.9%, 60.2% and 83.0%, respectively). The analysis of IgG avidity indices (AI) demonstrated the highest performance among the flow cytometry-based methods (Se = 96.6%; Sp = 93.3%), underscoring the low avidity index (AI < 60%) within TOXO (97.0%) in contrast with the high avidity index (AI > 60%) in NI (93%). Analysis of anti-T. gondii IgG and IgG3 reactivity for mother:infant paired samples may represent a relevant complementary tests for early diagnosis. In conclusion, afeasible high-standard algorithm (Accuracy = 97.1%) was proposed consisting of Q-Preven™ IgM screening at birth, followed by ELFAVIDAS™ IgM and flow cytometric IgG avidity analysis at 30–45 days after birth as a highperformance tool for early serological diagnosis of congenital toxoplasmosis. |
| Subject: | Citometria de Fluxo Imunoglobulina G |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICB - DEPARTAMENTO DE MICROBIOLOGIA ICB - DEPARTAMENTO DE PARASITOLOGIA MED - DEPARTAMENTO DE CLÍNICA MÉDICA MED - DEPARTAMENTO DE OFTALMOLOGIA E OTORRINOLARINGOLOGIA |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | 10.1016/j.jim.2017.08.005 |
| URI: | http://hdl.handle.net/1843/55299 |
| Issue Date: | 18-Aug-2017 |
| metadata.dc.url.externa: | https://www.sciencedirect.com/science/article/pii/S002217591730282X?via%3Dihub |
| metadata.dc.relation.ispartof: | Journal of Immunological Methods |
| Appears in Collections: | Artigo de Periódico |
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