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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/55299
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dc.creatorAlinede Castro Zacche-toninipt_BR
dc.creatorDaniel Vítor de Vasconcelos Santospt_BR
dc.creatorJosé Nélio Januáriopt_BR
dc.creatorAndréa Teixeira-carvalhopt_BR
dc.creatorRicardo Wagner Almeida Vitorpt_BR
dc.creatorEloísa Amália Vieira Ferropt_BR
dc.creatorJosé Roberto Mineopt_BR
dc.creatorOlindo Assis Martins-filhopt_BR
dc.creatorElenice Moreira Lemospt_BR
dc.creatorGiuliana Schmidt França Fonsecapt_BR
dc.creatorLaura Néspoli Nassar Passini de Jesuspt_BR
dc.creatorGeisa Baptista Barrospt_BR
dc.creatorJordana Grazziela Alves Coelho-dos-reispt_BR
dc.creatorSamantha Ribeiro Bélapt_BR
dc.creatorAnderson Silva Machadopt_BR
dc.creatorAna Carolina Aguiar Vasconcelos Carneiropt_BR
dc.creatorGláucia Manzan Queiroz Andradept_BR
dc.date.accessioned2023-06-23T22:22:32Z-
dc.date.available2023-06-23T22:22:32Z-
dc.date.issued2017-08-18-
dc.citation.volume451pt_BR
dc.citation.issue2017pt_BR
dc.citation.spage37pt_BR
dc.citation.epage47pt_BR
dc.identifier.doi10.1016/j.jim.2017.08.005pt_BR
dc.identifier.issn00221759pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/55299-
dc.description.resumoThe aim of this study was to evaluate the performance of conventional serology (Q-Preven™ and ELFAVIDAS™)and flow cytometry-based serologic tools for early serologic diagnosis of congenital toxoplasmosis. The study groups included prospectively confirmed cases of congenital toxoplasmosis (TOXO = 88) and age-matching non infected controls (NI = 15).The results demonstrated that all samples tested positive/indeterminate for anti-T.gondii IgM screening at birth using air-dried whole blood samples. Serum samples collected at 30–45 days after birth tested positive for ELFAVIDAS™ IgG in both groups. While all NI tested negative for ELFAVIDAS™ IgM and IgA, only 78% and 36% of TOXO tested positive for IgM and IgA, respectively. Flow cytometry-based anti-T. gondii IgM, IgA and IgG reactivity displayed moderate performance with low sensitivity (47.6%, 72.6% and 75.0%, respectively). Regardless the remarkable specificity of IgG1, IgG2 and IgG3 subclasses for early diagnosis,weak or moderate specificity was observed (Se = 73.9%, 60.2% and 83.0%, respectively). The analysis of IgG avidity indices (AI) demonstrated the highest performance among the flow cytometry-based methods (Se = 96.6%; Sp = 93.3%), underscoring the low avidity index (AI < 60%) within TOXO (97.0%) in contrast with the high avidity index (AI > 60%) in NI (93%). Analysis of anti-T. gondii IgG and IgG3 reactivity for mother:infant paired samples may represent a relevant complementary tests for early diagnosis. In conclusion, afeasible high-standard algorithm (Accuracy = 97.1%) was proposed consisting of Q-Preven™ IgM screening at birth, followed by ELFAVIDAS™ IgM and flow cytometric IgG avidity analysis at 30–45 days after birth as a highperformance tool for early serological diagnosis of congenital toxoplasmosis.pt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIApt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE PARASITOLOGIApt_BR
dc.publisher.departmentMED - DEPARTAMENTO DE CLÍNICA MÉDICApt_BR
dc.publisher.departmentMED - DEPARTAMENTO DE OFTALMOLOGIA E OTORRINOLARINGOLOGIApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Immunological Methods-
dc.rightsAcesso Restritopt_BR
dc.subjectCongenital T. gondii infectionpt_BR
dc.subjectFlow cytometrypt_BR
dc.subjectSerological diagnosispt_BR
dc.subjectIgGpt_BR
dc.subject.otherCitometria de Fluxopt_BR
dc.subject.otherImunoglobulina Gpt_BR
dc.titleEstablishing tools for early diagnosis of congenital toxoplasmosis: flow cytometric igg avidity assay as a confirmatory test for neonatal screeningpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.sciencedirect.com/science/article/pii/S002217591730282X?via%3Dihubpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0912-3782pt_BR
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