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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/55303
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dc.creatorGrazielle Ribeiro Goespt_BR
dc.creatorPeter Silva Rochapt_BR
dc.creatorAline r. s. Dinizpt_BR
dc.creatorPedro Henrique Nascimento de Aguiarpt_BR
dc.creatorCarlos Renato Machadopt_BR
dc.creatorLeda Quercia Vieirapt_BR
dc.date.accessioned2023-06-23T22:46:12Z-
dc.date.available2023-06-23T22:46:12Z-
dc.date.issued2016-
dc.citation.volume10pt_BR
dc.citation.issue4pt_BR
dc.identifier.doihttps://doi.org/10.1371/journal.pntd.0004555pt_BR
dc.identifier.issn1935-2735pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/55303-
dc.description.resumoBackground: During Trypanosoma cruzi infection, macrophages produce reactive oxygen species (ROS) in a process called respiratory burst. Several works have aimed to elucidate the role of ROS during T. cruzi infection and the results obtained are sometimes contradictory. T. cruzi has a highly efficiently regulated antioxidant machinery to deal with the oxidative burst, but the parasite macromolecules, particularly DNA, may still suffer oxidative damage. Guanine (G) is the most vulnerable base and its oxidation results in formation of 8-oxoG, a cellular marker of oxidative stress. Methodology/Principal Findings: In order to investigate the contribution of ROS in T. cruzi survival and infection, we utilized mice deficient in the gp91phox (Phox KO) subunit of NADPH oxidase and parasites that overexpress the enzyme EcMutT (from Escherichia coli) or TcMTH (from T. cruzi), which is responsible for removing 8-oxo-dGTP from the nucleotide pool. The modified parasites presented enhanced replication inside murine inflammatory macrophages from C57BL/6 WT mice when compared with control parasites. Interestingly, when Phox KO macrophages were infected with these parasites, we observed a decreased number of all parasites when compared with macrophages from C57BL/6 WT. Scavengers for ROS also decreased parasite growth in WT macrophages. In addition, treatment of macrophages or parasites with hydrogen peroxide increased parasite replication in Phox KO mice and in vivo. Conclusions: Our results indicate a paradoxical role for ROS since modified parasites multiply better inside macrophages, but proliferation is significantly reduced when ROS is removed from the host cell. Our findings suggest that ROS can work like a signaling molecule, contributing to T. cruzi growth inside the cells.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofPLoS Neglected Tropical Diseasespt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectTrypanosoma cruzipt_BR
dc.subjectChagas diseasept_BR
dc.subjectespécies reativas de nitrogêniopt_BR
dc.subjectespécies reativas de oxigêniopt_BR
dc.subject.otherTrypanosoma cruzipt_BR
dc.subject.otherDoença de chagaspt_BR
dc.subject.otherEspécies reativas de nitrogêniopt_BR
dc.subject.otherEspécies reativas de oxigêniopt_BR
dc.titleTrypanosoma cruzi needs a signal provided by reactive oxygen species to infect macrophagespt_BR
dc.title.alternativeTrypanosoma cruzi precisa de um sinal fornecido por espécies reativas de oxigênio para infectar macrófagospt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004555pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4942-5021pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-8724-3165pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-5481-9618pt_BR
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