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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/55308
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dc.creatorOlga Maria Pérez Carrascalpt_BR
dc.creatorNicolas Tromaspt_BR
dc.creatorYves Terratpt_BR
dc.creatorElisa Morenopt_BR
dc.creatorAlessandra Gianipt_BR
dc.creatorLaisa Corrêa Braga Marquespt_BR
dc.creatorNathalie Fortinpt_BR
dc.creatorJesse Shapiropt_BR
dc.date.accessioned2023-06-23T22:58:18Z-
dc.date.available2023-06-23T22:58:18Z-
dc.date.issued2021-
dc.citation.volume9pt_BR
dc.citation.issue1pt_BR
dc.citation.spage1pt_BR
dc.citation.epage21pt_BR
dc.identifier.doihttps://doi.org/10.1186/s40168-021-01140-8pt_BR
dc.identifier.issn2049-2618pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/55308-
dc.description.resumoBackground: Cyanobacteria from the genus Microcystis can form large mucilaginous colonies with attached heterotrophic bacteria—their microbiome. However, the nature of the relationship between Microcystis and its microbiome remains unclear. Is it a long-term, evolutionarily stable association? Which partners benefit? Here we report the genomic diversity of 109 individual Microcystis colonies—including cyanobacteria and associated bacterial genomes—isolated in situ and without culture from Lake Champlain, Canada and Pampulha Reservoir, Brazil. Results: We identified 14 distinct Microcystis genotypes from Canada, of which only two have been previously reported, and four genotypes specific to Brazil. Microcystis genetic diversity was much greater between than within colonies, consistent with colony growth by clonal expansion rather than aggregation of Microcystis cells. We also identified 72 bacterial species in the microbiome. Each Microcystis genotype had a distinct microbiome composition, and more closely related genotypes had more similar microbiomes. This pattern of phylosymbiosis could be explained by co-phylogeny in only two out of the nine most prevalent associated bacterial genera, Roseomonas and Rhodobacter. These phylogenetically associated genera could enrich the metabolic repertoire of Microcystis, for example by encoding the biosynthesis of complementary carotenoid molecules. In contrast, other colony-associated bacteria showed weaker signals of co-phylogeny, but stronger evidence of horizontal gene transfer with Microcystis. These observations suggest that acquired genes are more likely to be retained in both partners (Microcystis and members of its microbiome) when they are loosely associated, whereas one gene copy is sufficient when the association is physically tight and evolutionarily long-lasting.Conclusions: We have introduced a method for culture-free isolation of single colonies from nature followed by metagenomic sequencing, which could be applied to other types of microbes. Together, our results expand the known genetic diversity of both Microcystis and its microbiome in natural settings, and support their long-term, specific, and potentially beneficial associations.pt_BR
dc.languageporpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE BOTÂNICApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofMicrobiomept_BR
dc.rightsAcesso Abertopt_BR
dc.subjectMicrocystispt_BR
dc.subjectCyanobacteriapt_BR
dc.subjectPhylosymbiosispt_BR
dc.subjectCo-phylogenypt_BR
dc.subjectMicrobiomept_BR
dc.subjectPhycospherept_BR
dc.subject.otherCianobacteriapt_BR
dc.titleSingle-colony sequencing reveals microbe-by-microbiome phylosymbiosis between the cyanobacterium Microcystis and its associated bacteriapt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-021-01140-8pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-3442-6192pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-4332-8463pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-2524-8534pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-3653-0631pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-2966-0611pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-6819-8699pt_BR
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