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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/56317
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dc.creatorFranciele Martins Santospt_BR
dc.creatorCyntia Canedo Silvapt_BR
dc.creatorSergio Oliveira de Paulapt_BR
dc.creatorRoberto Souza Diaspt_BR
dc.creatorMichelle Dias Oliveirapt_BR
dc.creatorIsabella Cristina Toledo Alves Costapt_BR
dc.creatorLuciana Souza Fernandespt_BR
dc.creatorCarine Ribeiro Pessoapt_BR
dc.creatorSérgio Luís Pinto da Mattapt_BR
dc.creatorVivian Vasconcelos Costapt_BR
dc.creatorDanielle da Glória de Souzapt_BR
dc.date.accessioned2023-07-14T22:16:35Z-
dc.date.available2023-07-14T22:16:35Z-
dc.date.issued2019-
dc.citation.volume13pt_BR
dc.citation.issue5pt_BR
dc.citation.spage1pt_BR
dc.citation.epage20pt_BR
dc.identifier.doihttps://doi.org/10.1371/journal.pntd.0007375pt_BR
dc.identifier.issn1935-2735pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/56317-
dc.description.resumoBackground The Mayaro virus (MAYV) is an endemic arbovirus in South American countries, where it is responsible for sporadic outbreaks of Mayaro fever. Clinical manifestations include fever, headache, ocular pain, rash, myalgia, and debilitating and persistent polyarthralgia. Understanding the mechanisms associated with MAYV-induced arthritis is of great importance due to the potential for its emergence, urbanization and dispersion to other regions. Methods 15-day old Balb/c mice were infected by two distinct pathways, below the forelimb and in the rear footpad. Animals were observed for a period of 21 days. During this time, they were monitored every 24 hours for disease signs, such as weight loss and muscle weakness. Histological damage in the muscles and joints was evaluated 3, 7, 10, 15 and 20 days post-infection. The cytokine profile in serum and muscles during MAYV infection was evaluated by flow cytometry at different post-infection times. For pain analysis, the animals were submitted to the von Frey test and titre in different organs was evaluated throughout the study to obtain viral kinetics. Findings Infection by two distinct pathways, below the forelimb and in the rear footpad, resulted in a homogeneous viral spread and the development of acute disease in animals. Clinical signs were observed such as ruffled fur, hunched posture, eye irritation and slight gait alteration. In the physical test, both groups presented loss of resistance, which was associated with histopathological damage, including myositis, arthritis, tenosynovitis and periostitis. The immune response was characterized by a strong inflammatory response mediated by the cytokines TNF-α, IL-6 and INF-γ and chemokine MCP-1, followed by the action of IL-10 and IL-4 cytokines. Interpretation The results showed that Balb/c mice represent a promising model to study mechanisms involved in MAYV pathogenesis and for future antiviral testing.pt_BR
dc.languageporpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIApt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofPLOS Neglected Tropical Diseasespt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectMayaro virus (MAYV)pt_BR
dc.subjectSouth Americanpt_BR
dc.subjectClinical manifestationspt_BR
dc.subjectInfectionpt_BR
dc.subject.otherAmérica do Sulpt_BR
dc.subject.otherInfecçãopt_BR
dc.titleAnimal model of arthritis and myositis induced by the Mayaro viruspt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0007375pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-7102-673Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0375-8058pt_BR
dc.identifier.orcidhttp://orcid.org/0000-0003-1632-2058pt_BR
dc.identifier.orcidhttp://orcid.org/0000-0003-4327-9454pt_BR
dc.identifier.orcidhttp://orcid.org/0000-0002-3789-5907pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0175-642Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-3432-0769pt_BR
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