Please use this identifier to cite or link to this item:
http://hdl.handle.net/1843/56331| Type: | Artigo de Periódico |
| Title: | Characterization of aspergillus fumigatus axtracellular vesicles and their effects on macrophages and neutrophils functions |
| Authors: | Jéssica Amanda Marques Souza Frédéric Jean Georges Frézard Daniele da Glória de Souza Mauro Martins Teixeira Frederico Marianetti Soriani Ludmila de Matos Baltazar Virgínia Mendes Carregal Ludmila Gouveia Eufrasio André Gustavo de Oliveira Wendell Girard Dias Kildare Rocha de Miranda Iran Malavazi Daniel de Assis Santos |
| Abstract: | Extracellular vesicles (EVs) has been considered an alternative process for intercellular communication. EVs release by filamentous fungi and the role of vesicular secretion during fungus-host cells interaction remain unknown. Here, we identified the secretion of EVs from the pathogenic filamentous fungus, Aspergillus fumigatus. Analysis of the structure of EVs demonstrated that A. fumigatus produces round shaped bilayer structures ranging from 100 to 200 nm size, containing ergosterol and a myriad of proteins involved in REDOX, cell wall remodeling and metabolic functions of the fungus. We demonstrated that macrophages can phagocytose A. fumigatus EVs. Phagocytic cells, stimulated with EVs, increased fungal clearance after A. fumigatus conidia challenge. EVs were also able to induce the production of TNF-α and CCL2 by macrophages and a synergistic effect was observed in the production of these mediators when the cells were challenged with the conidia. In bone marrow-derived neutrophils (BMDN) treated with EVs, there was enhancement of the production of TNF-α and IL-1β in response to conidia. Together, our results demonstrate, for the first time, that A. fumigatus produces EVs containing a diverse set of proteins involved in fungal physiology and virulence. Moreover, EVs are biologically active and stimulate production of inflammatory mediators and fungal clearance. |
| Subject: | Aspergillus fumigatus Macrófagos Neutrofilos |
| language: | por |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA ICB - DEPARTAMENTO DE FARMACOLOGIA ICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA ICB - DEPARTAMENTO DE MICROBIOLOGIA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.3389/fmicb.2019.02008 |
| URI: | http://hdl.handle.net/1843/56331 |
| Issue Date: | 2019 |
| metadata.dc.url.externa: | https://www.frontiersin.org/articles/10.3389/fmicb.2019.02008/full |
| metadata.dc.relation.ispartof: | Frontiers in Microbiology |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Characterization of Aspergillus fumigatus Extracellular Vesicles and Their Effects on Macrophages and Neutrophils Functions.pdf | 2.07 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.