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Type:	Artigo de Periódico
Title:	Miniaturized Spectrophotometric Method for Quantification of Tannins in Pomegranate (Punica granatum L.) Fruit Peel Dried Extracts
Authors:	Juliana Machado Brêtas Diego Beltrão Pereira Isabela da Costa César Gerson Antônio Pianetti
Abstract:	Abstract: Background : Punica granatum L. (pomegranate) is one of the oldest edible fruits used for the treatment of countless diseases. The bioactivity of this fruit is mainly related to tannins, especially elagitannins. P.granatum fruit peel dried extracts (PPDEs) are widely marketed today and the lack of accessible quality control methods is a key to the variable and questionable quality of these products. Objec tive : To develop a miniaturized spectrophotometric method for quantification of tannins in commercial PPDEs. Me thod : Spectrophotometric method using Folin-Ciocalteu reagent was developed and validated for quantification of total polyphenols and polyphenols not adsorbed by hide powder in 17 samples of commercial PPDEs. Tannins content was calculated by the difference between total polyphenols and polyphenols not adsorbed by hide powder contents and expressed as pyrogallol. The UV/Visible microplate spectrophotometer was operated at 760 nm. Results : The developed miniaturized method was precise, accurate, selective and linear, and has no rotational or translational matrix effect. This method significantly reduced the cost of the analysis: 82.2% with reagents, 84.7% in electricity consumption, and 56.3% in the total analysis time. The wide variability in tannins contents in commercial PPDEs (<1% to 14.20%) shows the importance of quality control, ensuring the therapeutic effect and safety of the extracts. Conc lusion : A miniaturized spectrophotometric method was successfully developed and fully validated, being appropriate for quantification of tannins of PPDEs in quality control routine analysis, as it showed to be a simple and cheaper method.
Subject:	Brasil Controle de qualidade
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	FAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS FARMACIA - FACULDADE DE FARMACIA
Rights:	Acesso Restrito
metadata.dc.identifier.doi:	http://doi.org/10.2174/1573411015666181204115035
URI:	http://hdl.handle.net/1843/56500
Issue Date:	2020
metadata.dc.url.externa:	https://www.eurekaselect.com/article/94998
metadata.dc.relation.ispartof:	Current Analytical Chemistry
Appears in Collections:	Artigo de Periódico

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