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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/57516
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dc.creatorRicardo Adriano Dorledo de Fariapt_BR
dc.creatorPedro Henrique Cabral de Souzapt_BR
dc.creatorVanessa de Freitas Cunha Linspt_BR
dc.creatorTulio Matenciopt_BR
dc.creatorLuiz Guilherme Dias Heneinept_BR
dc.date.accessioned2023-08-07T13:09:19Z-
dc.date.available2023-08-07T13:09:19Z-
dc.date.issued2020-
dc.citation.volume25pt_BR
dc.citation.issue3pt_BR
dc.citation.spage19083pt_BR
dc.citation.epage19091pt_BR
dc.identifier.doihttps://doi.org/10.26717/BJSTR.2020.25.004190pt_BR
dc.identifier.issn2574-1241pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/57516-
dc.description.resumoTwo label-free impedimetric immunosensors were developed to be employed in the specific diagnosis of snakebite. To produce the devices, a conductive substrate was obtained by electrodeposition of Polyaniline on the surface of a Crofer 22 APU steel. The polymer-based substrates were functionalized either with an affinity purified antibody specific for venom of the Bothrops genus or for the Crotalus genus. The performance of the as-prepared sensors was evaluated by exposing them to different concentrations of the venoms from Bothrops, Crotalus and Micrurus snakes. The capacity to recognize the specific analytes was measure by Electrochemical Impedance Spectroscopy within a frequency range from 10,000 Hz to 0.01 Hz. The increase of the immunosensors’ charge transfer resistance was correlated to the increase in the concentration of the homologous venoms pool with a detection limit of 0.1 μg. m L-¹. Heterologous venoms were not detected at any tested concentration, proving the expected high selectivity. Furthermore, the results showed the reusability of the sensor, as it retained the capability of detecting the specific analytes after many regeneration cycles. The use of immunosensors to detect snake venom shows important promise as an aid to snakebite treatment.pt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorshipOutra Agênciapt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentENG - DEPARTAMENTO DE ENGENHARIA QUÍMICApt_BR
dc.publisher.departmentICX - DEPARTAMENTO DE QUÍMICApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofBiomedical Journal of Scientific & Technical Researchpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectImmunosensorpt_BR
dc.subjectElectrochemical impedance spectroscopypt_BR
dc.subjectPolyanilinept_BR
dc.subjectSnake venompt_BR
dc.subject.otherEletroquímicapt_BR
dc.subject.otherBiossensorespt_BR
dc.subject.otherEspectroscopia de impedânciapt_BR
dc.subject.otherCobra venenosa - Venenopt_BR
dc.subject.otherEletrodeposiçãopt_BR
dc.subject.otherPolímeros condutorespt_BR
dc.subject.otherReações antígeno-anticorpopt_BR
dc.titleLabel-free impedimetric immunosensors for detection of snake venoms using polyaniline as a transducer substratept_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://biomedres.us/fulltexts/BJSTR.MS.ID.004190.phppt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-7025-7042pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-2071-9309pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-6357-9553pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-5660-8125pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-0707-2691pt_BR
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