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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/57764
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dc.creatorV. H. de Almeidapt_BR
dc.creatorC. G. Ferreirapt_BR
dc.creatorC. Sternbergpt_BR
dc.creatorA. C. de Melopt_BR
dc.creatorD. D. Meirapt_BR
dc.creatorA. C. Pirespt_BR
dc.creatorAngélica Nogueira Rodriguespt_BR
dc.creatorH. K. Pimenta-Inadapt_BR
dc.creatorF. G. Alvespt_BR
dc.creatorG. Moralezpt_BR
dc.creatorL. S. Thiagopt_BR
dc.date.accessioned2023-08-11T20:14:27Z-
dc.date.available2023-08-11T20:14:27Z-
dc.date.issued2017-08-15-
dc.citation.volume51pt_BR
dc.citation.issue1pt_BR
dc.citation.spagee6822pt_BR
dc.citation.epagee6830pt_BR
dc.identifier.doihttps://doi.org/10.1590/1414-431X20176822pt_BR
dc.identifier.issn0100-879Xpt_BR
dc.identifier.urihttp://hdl.handle.net/1843/57764-
dc.description.resumoCervical cancer is a public health problem and the molecular mechanisms underlying radioresistance are still poorly understood.Here, we evaluated the modulation of key molecules involved in cell proliferation, cell cycle and DNA repair in cervical cancer lines (CASKI and C33A) and in malignant tissues biopsied from 10 patients before and after radiotherapy. The expression patterns of epidermal growth factor receptor (EGFR), excision repair cross-complementation group 1 (ERCC1) and p53 were evaluated in cancer cell lines by quantitative PCR and western blotting, and in human malignant tissues by immuno histochemistry. The mutation status of TP53 gene was evaluated by direct sequencing. Among cell lines, absent or weak modulations of EGFR, ERCC1 and p53 were observed after exposure to 1.8 Gy. Conversely, increased expressions of p53 (5/10 patients; P=0.0239), ERCC1 (5/10 patients; P=0.0294) and EGFR (4/10 patients; P=0.1773) were observed in malignant tissues after radiotherapy with the same radiation dose. TP53 mutations were found only in one patient. Here we show that a single dose of radiotherapy induced EGFR, ERCC1 and p53 expression in malignant tissues from cervical cancer patients but not in cancer cell lines, highlighting the gap between in vitro and in vivo experimental models. Studies on larger patient cohorts are needed to allow an interpretation that an upregulation of p53, EGFR and ERCC1 may be part of a radioresistance mechanism.pt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentMED - DEPARTAMENTO DE CLÍNICA MÉDICApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofBrazilian Journal of Medical and Biological Research-
dc.rightsAcesso Abertopt_BR
dc.subjectCervical cancerpt_BR
dc.subjectRadiotherapy modulatespt_BR
dc.subjectEpidermal growth factor receptor (EGFR)pt_BR
dc.subjectERCC1pt_BR
dc.subject.otherCervical cancerpt_BR
dc.subject.otherReceptores ErbBpt_BR
dc.subject.otherRadioterapia de Intensidade Moduladapt_BR
dc.subject.otherProteína Grupo D do Xeroderma Pigmentosopt_BR
dc.titleRadiotherapy modulates expression of EGFR, ERCC1 and p53 in cervical cancerpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.scielo.br/j/bjmbr/a/pgyXff8SCkzBhb8MRY8CzFq/?lang=enpt_BR
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