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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/59243
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dc.creatorGüínever Eustáquio do Impériopt_BR
dc.creatorTania Maria Ortiga Carvalhopt_BR
dc.creatorEnrrico Bloisept_BR
dc.creatorMohsen Javampt_BR
dc.creatorPhetcharawan Lyept_BR
dc.creatorAndrea Constantinofpt_BR
dc.creatorCaroline E. Dunkpt_BR
dc.creatorFernando Marcos Dos Reispt_BR
dc.creatorStephen Lyept_BR
dc.creatorWilliam Gibbpt_BR
dc.creatorStephen Matthewspt_BR
dc.date.accessioned2023-10-05T23:04:41Z-
dc.date.available2023-10-05T23:04:41Z-
dc.date.issued2019-
dc.citation.volume23pt_BR
dc.citation.issue1pt_BR
dc.citation.spage610pt_BR
dc.citation.epage618pt_BR
dc.identifier.doihttps://doi.org/10.1111/jcmm.13966pt_BR
dc.identifier.issn1582-1838pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/59243-
dc.description.resumoThe ATP-binding cassette (ABC) transporters control placental transfer of several nutrients, steroids, immunological factors, chemicals, and drugs at the maternal-fetal interface. We and others have demonstrated a gestational age-dependent expression pattern of two ABC transporters, P-glycoprotein and breast cancer resistance protein throughout pregnancy. However, no reports have comprehensively elucidated the expression pattern of all 50 ABC proteins, comparing first trimester and term human placentae. We hypothesized that placental ABC transporters are expressed in a gestational-age dependent manner in normal human pregnancy. Using the TaqMan® Human ABC Transporter Array, we assessed the mRNA expression of all 50 ABC transporters in first (first trimester, n = 8) and third trimester (term, n = 12) human placentae and validated the resulting expression of selected ABC transporters using qPCR, Western blot and immunohistochemistry. A distinct gene expression profile of 30 ABC transporters was observed comparing first trimester vs. term placentae. Using individual qPCR in selected genes, we validated the increased expression of ABCA1 (P < 0.01), ABCA6 (P < 0.001), ABCA9 (P < 0.001) and ABCC3 (P < 0.001), as well as the decreased expression of ABCB11 (P < 0.001) and ABCG4 (P < 0.01) with advancing gestation. One important lipid transporter, ABCA6, was selected to correlate protein abundance and characterize tissue localization. ABCA6 exhibited increased protein expression towards term and was predominantly localized to syncytiotrophoblast cells. In conclusion, expression patterns of placental ABC transporters change as a function of gestational age. These changes are likely fundamental to a healthy pregnancy given the critical role that these transporters play in the regulation of steroidogenesis, immunological responses, and placental barrier function and integrity.pt_BR
dc.languageporpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentMED - DEPARTAMENTO DE GINECOLOGIA OBSTETRÍCIApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Cellular and Molecular Medicinept_BR
dc.rightsAcesso Abertopt_BR
dc.subjectABC transporterspt_BR
dc.subjectABCA6pt_BR
dc.subjectGestational-agept_BR
dc.subjectLipid transporterpt_BR
dc.subjectPlacentapt_BR
dc.subject.otherPlacentapt_BR
dc.subject.otherTransportadores de Cassetes de Ligação de ATPpt_BR
dc.titleGestational age‐dependent gene expression profiling of ATP‐binding cassette transporters in the healthy human placentapt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://onlinelibrary.wiley.com/doi/full/10.1111/jcmm.13966pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-5744-6623pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-0275-8686pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4146-099Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-3305-4406pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-5063-1773pt_BR
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