Extrato etanólico e filtrado do cultivo de Aspergillus terreus na eclodibilidade e desenvolvimento de nematódeos gastrintestinais de ovinos e bovinos

Abstract

The objective of this study was to evaluate the in vitro efficacy of the filtrate and ethanol extract of Aspergillus terreus isolates in inhibiting the hatchability (IE) and larval development (IDL) of Haemonchus contortus. For the evaluation of IE, after 72 hours of incubation with filtrate (1, 2, 3.01 and 4.02 mg/ mℓ) and ethanol extract (17.31, 34.62, 51.93 and 69.25 mg/ mℓ), non-embryonic eggs, larvae and infective first stage larvae (L1) were quantified. Sterile distilled water was used as a negative control and 15 mg/mℓ of evamisole phosphate as a positive control. For IDL, the adapted method of quantitative stool culture was used. After seven days of incubation of feces with different concentrations of fungal filtrates, the infecting larvae were collected and quantified, obtaining the number of larvae developed per gram of feces (LDGP). 15 mg/mℓ of ivermectin or distilled water were used as controls. In IE, the ethanol extract of Aspergillus terreus, at a concentration of 69.25 mg/mℓ, showed 100% efficacy. As for the IDL, concentrations equal to or greater than 34.62 mg/mℓ presented results similar to the positive control (levamisol). In cattle, the ethanol extract showed 100% efficiency at all levels tested on different genera of gastrointestinal parasites. It is concluded that the ethanol filtrates and extracts of the fungi evaluated in this paper have potential for the alternative control of intestinal parasites.