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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/59413
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dc.creatorJoão Victor Coelho Pimentapt_BR
dc.creatorAdão Aparecido Sabinopt_BR
dc.creatorRodinei Augustipt_BR
dc.date.accessioned2023-10-11T20:21:02Z-
dc.date.available2023-10-11T20:21:02Z-
dc.date.issued2022-
dc.citation.volume479pt_BR
dc.citation.spage116876pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.ijms.2022.116876pt_BR
dc.identifier.issn1387-3806pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/59413-
dc.description.resumoPaper spray mass spectrometry (PS-MS) is a recent and innovative ambient ionization technique conveniently applied to investigate on-surface chemical reactions. The easy, fast, and efficient PS-MS technique has shown to be a convenient platform to investigate organic reactions, yielding promising results. Lipases are a group of enzymes responsible for esterification and hydrolysis reactions of organic analytes, such as carbohydrates and lipids. Hence, this work describes for the first time an on-surface enzymatic reaction monitored by PS-MS: the deacetylation of 1,2,3,4-tetra-O-acetyl-β-D-xylopyranose (1) induced by the enzyme Burkholderia cepacia lipase (BCL). The reaction was followed over 60 min when the sodium monodeacetylated product (including the respective sodium-bound dimer) and the oxonium intermediate were detected at high intensities. Besides, the influence of BCL concentration and the presence of water and hydrochloric acid were evaluated. Finally, the results described herein demonstrated the feasibility of applying PS-MS to evaluate and get information on on-surface enzymatic reactions, a field still underexplored. These results also open up a new and exciting perspective concerning studying other and perhaps more complex enzymatic reactions in a straightforward manner.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICX - DEPARTAMENTO DE QUÍMICApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofInternational Journal of Mass Spectrometrypt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectEnzymatic reactionnpt_BR
dc.subjectBCL enzymept_BR
dc.subjectMass spectrometrypt_BR
dc.subjectOn-surface reactionspt_BR
dc.subjectPaper spray ionizationpt_BR
dc.subjectNuclear magnetic resonancept_BR
dc.subject.otherAnálise enzimáticapt_BR
dc.subject.otherEnzimas - Análisept_BR
dc.subject.otherMacromoléculaspt_BR
dc.subject.otherEspectrometria de massapt_BR
dc.subject.otherLipasept_BR
dc.subject.otherRessonancia magnetica nuclearpt_BR
dc.subject.otherÁcido clorídricopt_BR
dc.subject.otherCatálisept_BR
dc.titleThe first example of an on-surface enzymatic reaction evaluated by paper spray mass spectrometry (ps-ms): deacetylation of 1,2,3,4-tetra-o-acetyl-β-d-xylopyranosept_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.sciencedirect.com/science/article/pii/S1387380622000811pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-1265-7694pt_BR
dc.identifier.orcidhttps://orcid.org/0009-0008-2944-5536pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-9448-9518pt_BR
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