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http://hdl.handle.net/1843/60148| Tipo: | Artigo de Periódico |
| Título: | Simultaneous saccharification isomerization and Co-fermentation - SSICF: a new process concept for second-generation ethanol biorefineries combining immobilized recombinant enzymes and non-GMO Saccharomyces |
| Autor(es): | Ederson Paulo Xavier Guilherme Leticia Maria Zanphorlin Amanda Silva Sousa Renan Yuji Miyamoto Carlos Giovani Oliveira Bruziquesi Bruna Mara Aparecida de Carvalho Mesquita Sergio Henrique Sousa Santos Elizama Aguiar-Oliveira Junio Cota Silva |
| Resumo: | Integrated bioprocess strategies may facilitate ethanol production from both C6 and C5 fractions of lignocellulosic feedstocks. We propose a new process concept, SSICF, where sugarcane bagasse is hydrolyzed simultaneously with xylose isomerization and the co-fermentation of C6 and C5 sugars. A commercial cocktail was supplemented with our multi-enzymatic system composed of three recombinant enzymes immobilized in Feroxyhyte magnetic nanoparticles: β-glucosidase, β-xylosidase and xylose isomerase. SSICF was performed using non-GMO Saccharomyces at pH 6.0 and 35 °C for 72 h in a synthetic medium containing cellobiose and xylose, and another medium containing pretreated sugarcane bagasse (PSB). The results of ethanol global yields in SSICF were 77.67% and 73.24% for the synthetic medium and PSB, respectively. In a nutshell, this is the first report of a successful proof-of-concept of SSICF with four rounds of enzyme recycling and a non-GMO yeast, an innovative process with high potential for industrial use. |
| Assunto: | Hidroxidos Óxidos Enzimas imobilizadas Fermentação Álcool Hidrolise |
| Idioma: | eng |
| País: | Brasil |
| Editor: | Universidade Federal de Minas Gerais |
| Sigla da Instituição: | UFMG |
| Departamento: | ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
| Tipo de Acesso: | Acesso Restrito |
| Identificador DOI: | https://doi.org/10.1016/j.renene.2021.10.023 |
| URI: | http://hdl.handle.net/1843/60148 |
| Data do documento: | 2022 |
| metadata.dc.url.externa: | https://www.sciencedirect.com/science/article/pii/S0960148121014804 |
| metadata.dc.relation.ispartof: | Renewable Energy |
| Aparece nas coleções: | Artigo de Periódico |
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