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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/60283
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dc.creatorKamila R. Santospt_BR
dc.creatorAngélica Rosa Fariapt_BR
dc.creatorHélida Monteiro de Andradept_BR
dc.creatorMônica M. O. P. Cerqueirapt_BR
dc.creatorMagnus Gidlundpt_BR
dc.creatorHiro Gotopt_BR
dc.creatorAlice Maria M. P. Della Liberapt_BR
dc.creatorFernando N. Souzapt_BR
dc.creatorEduardo M. Ramos-Sanchezpt_BR
dc.creatorCamila F. Batistapt_BR
dc.creatorLuiza C. Reispt_BR
dc.creatorWesley L. Fotoranpt_BR
dc.creatorMarcos B. Heinemannpt_BR
dc.creatorAdriano F. Cunhapt_BR
dc.creatorMussya C. Rochapt_BR
dc.date.accessioned2023-10-30T21:05:41Z-
dc.date.available2023-10-30T21:05:41Z-
dc.date.issued2022-10-02-
dc.citation.volume11pt_BR
dc.citation.issue12pt_BR
dc.citation.spage1831pt_BR
dc.citation.epage12pt_BR
dc.identifier.doihttps://doi.org/10.3390/antibiotics11121831pt_BR
dc.identifier.issn2079-6382pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/60283-
dc.description.resumoBackground: Staphylococcus aureus is one of the most frequently major mastitis pathogens that cause clinical and subclinical mastitis worldwide. Current antimicrobial treatments are usually ineffective, and the commercially available vaccines lack proven effectiveness. The immunological response elicited by the recombinant S. aureus-cure-associated proteins phosphoglycerate kinase (PGK), enolase (ENO), and elongation factor-G (EF-G) in combination with the granulocyte-macrophage colony-stimulating factor (GM-CSF) DNA vaccination was studied in this work. Methods: Here, twenty-three C57BL/6 mice were divided into four groups and vaccinated with: G1: none (control); G2: GM-CSF DNA plasmid DNA vaccine; G3: the combination of EF-G+ENO+PGK; and G4: the combinations of EF-G+ENO+PGK proteins plus GM-CSF plasmid DNA vaccine. After 44 days, spleen cells were collected for immunophenotyping and lymphocyte proliferation evaluation by flow cytometry upon S. aureus stimulus. Results: Immunization with the three S. aureus recombinant proteins alone resulted in a higher percentage of IL-17A+ cells among CD8+ T central memory cells, as well as the highest intensity of IL-17A production by overall lymphocytes indicating that the contribution of the combined lymphocyte populations is crucial to sustaining a type 3 cell immunity environment. Conclusion: The immunization with three S. aureus-cure-associated recombinant proteins triggered type 3 immunity, which is a highly interesting path to pursue an effective bovine S. aureus mastitis vaccine.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICASpt_BR
dc.publisher.departmentFARMACIA - FACULDADE DE FARMACIApt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE PARASITOLOGIApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofAntibioticspt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectVaccinept_BR
dc.subjectT cell responsept_BR
dc.subjectIL-17Apt_BR
dc.subjectIntramammary infectionpt_BR
dc.subjectStaphylococcus aureuspt_BR
dc.subjectMastitispt_BR
dc.subjectRecombinant antigenspt_BR
dc.subjectDairy cowpt_BR
dc.subject.otherVacinaçãopt_BR
dc.subject.otherCélulas T.pt_BR
dc.subject.otherInfecçõespt_BR
dc.subject.otherLaticíniospt_BR
dc.titleStaphylococcus aureus-Cure-Associated Antigens Elicit Type 3 Immune Memory T Cellspt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.mdpi.com/2079-6382/11/12/1831pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-9476-202Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-5380-0939pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8700-5469pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-5412-4066pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8203-8384pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-6444-9788pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-9118-8464pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-7246-2648pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-2047-1136pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-7048-562Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-5767-5920pt_BR
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