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http://hdl.handle.net/1843/61018| Type: | Artigo de Periódico |
| Title: | The Ecto-5′nucleotidase/CD73 Mediates Leishmania amazonensis Survival in Macrophages |
| Authors: | Bijay Bajracharya Deena Shrestha Andre Talvani Pedrosa da Silva Ricardo Gonçalves Luis Carlos Crocco Afonso |
| Abstract: | Endogenous nucleotides produced by various group of cells under inflammatory conditions act as potential danger signals in vivo. Extracellularly released nucleotides such as ATP are rapidly hydrolyzed to adenosine by the coordinated ectonucleotidase activities of CD39 and CD73. Leishmania is an obligate intracellular parasite of macrophages and capable of modulating host immune response in order to survive and multiply within host cells. In this study, the activity of CD73 induced by Leishmania amazonensis in infected macrophages has been investigated and correlated with parasite survival and infection in vitro. For this, the expression of CD39 and CD73, by flow cytometry, in murine peritoneal macrophages infected with metacyclic promastigotes of L. amazonensis has been analyzed. Our results showed that L. amazonensis-infected macrophages, unlike LPS-treated macrophages, increased CD73 expression. It was also noted that when CD73 enzymatic activity was blocked by α, β-methyleneadenosine 5-diphosphate sodium salt (APCP), macrophage parasitism was significantly decreased. Interestingly, these effects were not associated with the production of TNF-α, IL-10, or nitric oxide (NO). Together, these data demonstrate that L. amazonensis induces a regulatory phenotype in macrophages, which by activating the CD39/CD73 pathway allows parasite survival through the action of immunomodulatory adenosine receptors. |
| Subject: | Macrófagos Inflamação Leishmania |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICB - DEPARTAMENTO DE PATOLOGIA ICB - INSTITUTO DE CIÊNCIAS BIOLOGICAS |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.1155/2022/9928362 |
| URI: | http://hdl.handle.net/1843/61018 |
| Issue Date: | 11-Feb-2022 |
| metadata.dc.url.externa: | https://www.hindawi.com/journals/bmri/2022/9928362/ |
| metadata.dc.relation.ispartof: | BioMed Research International |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| The Ecto-5nucleotidase_CD73 Mediates Leishmania amazonensis Survival in Macrophages.pdf | 3.45 MB | Adobe PDF | View/Open |
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