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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/61249
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dc.creatorEdison Andrés Cruz Olivopt_BR
dc.creatorDaniel Santospt_BR
dc.creatorMaria Elena de Limapt_BR
dc.creatorVera Lúcia dos Santospt_BR
dc.creatorRubén Dario Sinisterra Millánpt_BR
dc.creatorMaría Esperanza Cortés Segurapt_BR
dc.date.accessioned2023-11-22T13:17:11Z-
dc.date.available2023-11-22T13:17:11Z-
dc.date.issued2017-06-
dc.citation.volume88pt_BR
dc.citation.issue6pt_BR
dc.citation.spagee88pt_BR
dc.citation.epagee96pt_BR
dc.identifier.doihttps://doi.org/10.1902/jop.2016.160438pt_BR
dc.identifier.issn1943-3670pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/61249-
dc.description.resumoBackground: Antimicrobial peptides (AMPs) have shown rapid and potent effect against planktonic bacteria. However, control of periodontopathic biofilms is a challenge even for AMPs. Thus, the present study evaluates in vitro antimicrobial activity of synthetic peptide LyeTxI and association compound LyeTxI/β-cyclodextrin (βCD) against multispecies biofilms. Methods: Sensibility to LyeTxI and LyeTxI/βCD was determined for planktonic Gram-negative periodontopathogens. Time-kill kinetic assay was performed at minimum inhibitory concentrations (MICs) in all planktonic strains. Multispecies biofilms were grown on pegs using a biofilm device and studied by scanning electron microscopy at 2, 5, and 10 days. Minimal biofilm eradication concentration (MBEC) was determined for 2- and 4-day multispecies biofilms. Metabolic activity of biofilms was determined by fluorometry study. Results: Biofilms showed reproducible cell density on pegs of the biofilm device. LyeTxI and LyeTxI/βCD were active against all strains tested at concentrations ≤62.5 μg/mL. Kinetic assays showed rapid bactericidal effect of LyeTxI against all periodontopathogens. MBECs of LyeTxI and LyeTxI/βCD against multispecies 2-day biofilms were two-fold higher than MICs of cells shed from biofilms. LyeTxI was able to reduce multispecies 2-day metabolic activity by 90%. Multispecies 4-day biofilms were tolerant to all agents tested. Conclusions: LyeTxI and LyeTxI/βCD are active against periodontopathic bacteria, showing rapid bactericidal effect and may be used to prevent biofilm development. In the future, AMPs could be therapeutic tools for treatment of periodontitis.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorshipOutra Agênciapt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORApt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIApt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIApt_BR
dc.publisher.departmentICX - DEPARTAMENTO DE QUÍMICApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Periodontologypt_BR
dc.rightsAcesso Restritopt_BR
dc.subjectAntimicrobial cationic peptidespt_BR
dc.subjectBiofilmspt_BR
dc.subjectCyclodextrinspt_BR
dc.subjectFusobacteriumpt_BR
dc.subjectPeriodontitispt_BR
dc.subjectPorphyromonaspt_BR
dc.subject.otherCiclodextrinaspt_BR
dc.subject.otherBiofilmept_BR
dc.subject.otherDoença periodontalpt_BR
dc.subject.otherAgentes antibacterianospt_BR
dc.subject.otherPeriodontitept_BR
dc.subject.otherMicroscopia eletrônica de varredurapt_BR
dc.subject.otherFluorimetriapt_BR
dc.titleAntibacterial effect of synthetic peptide LyeTxI and LyeTxI/ß-Cyclodextrin association compound against planktonic and multispecies biofilms of periodontal pathogenspt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://aap.onlinelibrary.wiley.com/doi/10.1902/jop.2016.160438pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-7416-3775pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-6185-4032pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-5815-5652pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-7656-1849pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0560-8491pt_BR
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