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http://hdl.handle.net/1843/61342| Type: | Artigo de Periódico |
| Title: | Enhanced efficacy against bacterial biofilms via host:guest cyclodextrin-doxycycline inclusion complexes |
| Authors: | Pedro Pires Goulart Guimarães Andressa Coelho de Menezes Karina Imaculada Rosa Teixeira Ângelo Márcio Leite Denadai Richard Alfonso Fills Cerchar María Esperanza Cortés Segura Rubén Dario Sinisterra Millán |
| Abstract: | Periodontal disease is characterized by a microbial infection and it is one of the major causes of teeth loss. The growth of pathogenic bacteria in oral cavity, such as Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), provides a favorable enviromment for the biofilm formation, which result in periodontal diseases. The development of new technologies to potentiate existing drugs, avoiding bacterial biofilms resistance and improving chemical stability is highly desirable. Here, we report the use of host–guest chemistry to enhance the activity of antibacterial doxycycline (DOX) in A. actinomycetemcomitans bacterial strain suspension and biofilm in vitro through complexation with hydroxypropyl-β-cyclodextrin (HPβCD) using different molar ratios of DOX/HPβCD. 2D ¹H NMR confirmed the host–guest complexation of DOX and HPβCD. We assessed the colloidal characteristics of the complex DOX/HPβCD via Dynamic Light Scattering (DLS) and Zeta Potential (PZ). The mixing ratio 1:2 DOX/HPβCD significantly decreased the minimum inhibitory concentration (MIC) and improved efficacy against A. actinomycetemcomitans suspensions and biofilms, respectively, when compared to free DOX and other DOX/HPβCD complexes. Further, the interaction of different molar ratio proportions of DOX/HPβCD complex with bacterial membrane was demonstrated via Isothermal Titration Calorimetry (ITC). Thus, we suggested the enhanced efficacy of the DOX/HPβCD complexes, at molar ratio 1:2, is due the higher cyclodextrin ratio, which potentiate the interaction between drug and bacterial membrane through nonionic interactions, such as hydrogen bonding or other van der Waals interactions. Collectively, the development of these complexes enables increased efficacy against bacterial biofilms, which hold promise for the treatment of aggressive and non-responsive forms of periodontitis. |
| Subject: | Ciclodextrinas Sistemas de distribuição de medicamentos Biofilme Doença periodontal Periodontite Agentes antibacterianos Luz - Espalhamento Potencial zeta Calorimetria |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA ICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA ICX - DEPARTAMENTO DE QUÍMICA |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | https://doi.org/10.1007/s10847-020-01041-7 |
| URI: | http://hdl.handle.net/1843/61342 |
| Issue Date: | Oct-2021 |
| metadata.dc.url.externa: | https://link.springer.com/article/10.1007/s10847-020-01041-7 |
| metadata.dc.relation.ispartof: | Journal of Inclusion Phenomena and Macrocyclic Chemistry |
| Appears in Collections: | Artigo de Periódico |
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