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http://hdl.handle.net/1843/61450| Type: | Artigo de Periódico |
| Title: | Bothrops moojeni snake peptide cyclodextrin complex: production, characterization and in vitro evaluation |
| Authors: | Karina Imaculada Rosa Teixeira Robson Augusto de Souza Santos Fabio de Oliveira Rubén Dario Sinisterra Millán María Esperanza Cortés Segura |
| Abstract: | The BmooMPα-I moojeni snake peptide was studied aimed to increase the therapeutic use as antitumor agent. Complexes were prepared and its physical characteristics were determined: particle size and Zeta-potential (ζ), antiproliferative and cytotoxic effects. BmooMPα-I peptide associated to Hp-βCD was able to change the cell surface parameters, such as ζ-potential which was negative. Hp-βCD:BmooMPα-I and BmooMPα-I alter the ζ of cells in contact with peptides and its association compound shown negative charge, that no favor the interaction with biological surfaces. In comparison the pure peptide as biocidal of epidermoid Carcinoma cells A431 or epithelial cells rather the fibroblast, the BmooMPα-I / Hp-βCD resulted in significant inhibition of Caco-2 human epithelial cancer cells (31.2 μg/mL after 24h), and Human Epidermoid Carcinoma cells A431(3.9 μg/mL after 24h), in a more efficient manner than peptide alone (p< 0.001), and was able to alter the degree of hemolysis. Next, the Lactate Dehydrogenase was examined to know the peptide cytotoxic effects and showed lower cytotoxic effects on EC50 for fibroblastic cells. Based on the BmooMPα-I /Hp-βCD properties observed and its ability of inhibiting the proliferation of epithelial cells, this study highlight that this compound is a biocidal with high potential as an anti-tumoral agent. |
| Subject: | Ciclodextrinas Carcinoma de células escamosas Agentes antineoplásicos Potencial zeta Sistemas de distribuição de medicamentos |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA ICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA ICX - DEPARTAMENTO DE QUÍMICA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.33263/LIANBS54.467474 |
| URI: | http://hdl.handle.net/1843/61450 |
| Issue Date: | 2016 |
| metadata.dc.url.externa: | https://nanobioletters.com/wp-content/uploads/2021/02/2284680853467474.pdf |
| metadata.dc.relation.ispartof: | Letters in Applied NanoBioScience |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Bothrops moojeni snake peptide cyclodextrin complex.pdf | 723.85 kB | Adobe PDF | View/Open |
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