Please use this identifier to cite or link to this item:
http://hdl.handle.net/1843/63211| Type: | Artigo de Periódico |
| Title: | Splenic implant preservation after conservation in lactated ringer solution |
| Other Titles: | Preservação de implante esplênico autógeno após conservação em solução de Ringer-lactato |
| Authors: | Argos Soares Matos Filho Andy Petroianu Valbert Nascimento Cardoso Paula Vieira Teixeira Vidigal |
| Abstract: | Objective: to evaluate the morphology and function of autogenous splenic tissue implanted in the greater omentum, 24 hours after storage in Ringer-lactate solution. Methods: we divided 35 male rats into seven groups (n=5): Group 1: no splenectomy; Group 2: total splenectomy without implant; Group 3: total splenectomy and immediate autogenous implant; Group 4: total splenectomy, preservation of the spleen in Ringer-lactate at room temperature, then sliced and implanted; Group 5: total splenectomy, spleen sliced and preserved in Ringer-lactate at room temperature before implantation; Group 6: total splenectomy with preservation of the spleen in Ringer lactate at 4°C and then sliced and implanted; Group 7: total splenectomy and the spleen sliced for preservation in Ringer-lactate at 4°C before implantation. After 90 days, we performed scintigraphic studies with Tc99m-colloidal tin (liver, lung, spleen or implant and clot), haematological exams (erythrogram, leucometry, platelets), biochemical dosages (protein electrophoresis) and anatomopathological studies. Results: regeneration of autogenous splenic implants occurred in the animals of the groups with preservation of the spleen at 4ºC. The uptake of colloidal tin was higher in groups 1, 3, 6 and 7 compared with the others. There was no difference in hematimetric values in the seven groups. Protein electrophoresis showed a decrease in the gamma fraction in the group of splenectomized animals in relation to the operated groups. Conclusion: the splenic tissue preserved in Ringer-lactate solution at 4ºC maintains its morphological structureand allows functional recovery after being implanted on the greater omentum |
| Subject: | Spleen Implants, Experimental Trauma and Stressor Related Disorders Organ Preservation |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | MED - DEPARTAMENTO DE ANATOMIA PATOLÓGICA E MEDICINA LEGAL MED - DEPARTAMENTO DE CIRURGIA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.1590/0100-6991e-20181346 |
| URI: | http://hdl.handle.net/1843/63211 |
| Issue Date: | 2018 |
| metadata.dc.url.externa: | https://doi.org/10.1590/0100-6991e-20181346 |
| metadata.dc.relation.ispartof: | Revista do Colégio Brasileiro de Cirurgiões |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Splenic implant preservation after conservation in lactated pdfa.pdf | 6.16 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.