Mesoscopic modelling of Cy3 and Cy5 dyes attached to DNA duplexes

Use este identificador para citar ou linkar para este item: http://hdl.handle.net/1843/64414

Tipo:	Artigo de Periódico
Título:	Mesoscopic modelling of Cy3 and Cy5 dyes attached to DNA duplexes
Autor(es):	Pâmella Miranda de Moura Luciana Márcia de Oliveira Gerald Weber
Resumo:	Cy3 and Cy5 dyes linked to the 5′ end of a double stranded DNA molecule are known to attach to both strands in a way that is very similar to an additional base pair and has a stabilizing effect on the oligonucleotide. Here we adapt the Peyrard-Bishop mesoscopic model to incorporate cyanine dyes and use the technique of thermal equivalence to obtain the appropriate parameters from existing melting temperatures. We have found that the stacking parameters are in the same range of ordinary AT and CG base pairs, in particular Cy3-A was found to be most rigidly stacked. While the cyanines stabilize the AT hydrogen bonds quite strongly the CG bonds are mostly unaffected.
Assunto:	Biofísica DNA Hidrogênio
Idioma:	eng
País:	Brasil
Editor:	Universidade Federal de Minas Gerais
Sigla da Instituição:	UFMG
Departamento:	ICX - DEPARTAMENTO DE FÍSICA
Tipo de Acesso:	Acesso Restrito
Identificador DOI:	https://doi.org/10.1016/j.bpc.2017.08.007
URI:	http://hdl.handle.net/1843/64414
Data do documento:	2017
metadata.dc.url.externa:	https://www.sciencedirect.com/science/article/pii/S0301462217302831?via%3Dihub
metadata.dc.relation.ispartof:	Biophysical Chemistry
Aparece nas coleções:	Artigo de Periódico

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